dc.creator | Galmozzi, Carla V. | es |
dc.creator | Muro Pastor, María Isabel | es |
dc.date.accessioned | 2019-02-06T07:49:50Z | |
dc.date.available | 2019-02-06T07:49:50Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Galmozzi, C.V. y Muro Pastor, M.I. (2017). Isolation of Ribosomal Particles from the Unicellular Cyanobacterium Synechocystis sp. PCC 6803. Bio-protocol, 7 (6), 1-10. | |
dc.identifier.issn | 2331-8325 | es |
dc.identifier.uri | https://hdl.handle.net/11441/82555 | |
dc.description.abstract | Isolation of ribosomal particles is an essential step in the study of ribosomal components as
well as in the analysis of trans-acting factors that interact with the ribosome to regulate protein synthesis
and modulate the expression profile of the cell in response to different environmental conditions. In this
protocol, we describe a procedure for the isolation of 70S ribosomes from the unicellular cyanobacterium
Synechocystis sp. PCC 6803 (hereafter Synechocystis). We have successfully used this protocol in our
study of the cyanobacterial ribosomal-associated protein LrtA, which is a homologue of bacterial HPF
(hibernation promoting factor) (Galmozzi et al., 2016). | es |
dc.description.sponsorship | España, Junta de Andalucía grant P07-CVI-02792 and group BIO-284 | es |
dc.description.sponsorship | España, MINECO y Fondo Social Europeo grant BFU2013-41712-P | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Bio-Protocol | es |
dc.relation.ispartof | Bio-protocol, 7 (6), 1-10. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Ribosome | es |
dc.subject | Sucrose gradients | es |
dc.subject | Synechocystis | es |
dc.subject | Cyanobacteria | es |
dc.subject | Photosynthetic prokaryotes | es |
dc.title | Isolation of Ribosomal Particles from the Unicellular Cyanobacterium Synechocystis sp. PCC 6803 | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | P07-CVI-02792 | es |
dc.relation.projectID | BFU2013-41712-P | es |
dc.relation.publisherversion | http://dx.doi.org/10.21769/BioProtoc.2176 | es |
dc.identifier.doi | 10.21769/BioProtoc.2176 | es |
idus.format.extent | 10 p. | es |
dc.journaltitle | Bio-protocol | es |
dc.publication.volumen | 7 | es |
dc.publication.issue | 6 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 10 | es |
dc.contributor.funder | Junta de Andalucía | |
dc.contributor.funder | Ministerio de Economia, Industria y Competitividad (MINECO). España | |