Artículo
The CBRB regulon: Promoter dissection reveals novel insights into the CbrAB expression network in Pseudomonas putida
Autor/es | Barroso, Rocío
García Mauriño, Sofía M. Tomás Gallardo, Laura Andújar, Eloísa Pérez Alegre, Mónica Santero Santurino, Eduardo Canosa, I. |
Departamento | Universidad de Sevilla. Departamento de Biología Vegetal y Ecología |
Fecha de publicación | 2018 |
Fecha de depósito | 2019-01-24 |
Publicado en |
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Resumen | CbrAB is a high ranked global regulatory system exclusive of the Pseudomonads that responds to carbon limiting conditions. It has become necessary to define the particular regulon of CbrB and discriminate it from the ... CbrAB is a high ranked global regulatory system exclusive of the Pseudomonads that responds to carbon limiting conditions. It has become necessary to define the particular regulon of CbrB and discriminate it from the downstream cascades through other regulatory components. We have performed in vivo binding analysis of CbrB in P. putida and determined that it directly controls the expression of at least 61 genes; 20% involved in regulatory functions, including the previously identified CrcZ and CrcY small regulatory RNAs. The remaining are porines or transporters (20%), metabolic enzymes (16%), activities related to protein translation (5%) and orfs of uncharacterised function (38%). Amongst the later, we have selected the operon PP2810-13 to make an exhaustive analysis of the CbrB binding sequences, together with those of crcZ and crcY. We describe the implication of three independent non-palindromic subsites with a variable spacing in three different targets; CrcZ, CrcY and operon PP2810-13 in the CbrAB activation. CbrB is a quite peculiar σN—depen-dent activator since it is barely dependent on phosphorylation for transcriptional activation. With the depiction of the precise contacts of CbrB with the DNA, the analysis of the multi-merisation status and its dependence on other factors such as RpoN o IHF, we propose a model of transcriptional activation. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España |
Identificador del proyecto | BIO2014-57545-R |
Cita | Barroso, R., García Mauriño, S.M., Tomás Gallardo, L., Andújar Pulido, E., Pérez Alegre, M., Santero, E. y Canosa, I. (2018). The CBRB regulon: Promoter dissection reveals novel insights into the CbrAB expression network in Pseudomonas putida. PLoS ONE, 13 (12), e0209191-. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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The CbrB Regulon.pdf | 1.833Mb | [PDF] | Ver/ | |