Different and new Nod factors produced by Rhizobium tropici CIAT899 following Na+ stress
|Author||Estévez Amador, Jana
Soria Díaz, María Eugenia
Córdoba, F. F. de
Gil Serrano, Antonio Miguel
Thomas-Oates, Jane E.
Van Brussel, Anton Albertus Nicolaas
Dardanelli, Marta Susana
Sousa Martín, Carolina
|Department||Universidad de Sevilla. Departamento de Microbiología y Parasitología
Universidad de Sevilla. Departamento de Química orgánica
|Published in||FEMS Microbiology Letters, 293 (2), 220-231.|
|Abstract||The root nodule bacterium Rhizobium tropici strain CIAT899 is highly stress resistant. It grows under acid conditions, in large amounts of salt, and at high osmotic pressure. An earlier study reported a substantial qualitative ...
The root nodule bacterium Rhizobium tropici strain CIAT899 is highly stress resistant. It grows under acid conditions, in large amounts of salt, and at high osmotic pressure. An earlier study reported a substantial qualitative and quantitative effect of acid stress on the biosynthesis of Nod factors. The aim of the present work was to investigate the effect of high salt (NaCl) concentrations, another common stress factor, on Nod factor production. For this purpose, thin-layer chromatography, HPLC and MS analyses were carried out. The expression of nodulation genes was also studied using a nodPlacZ fusion. High concentrations of sodium enhanced nod gene expression and Nod factor biosynthesis. The effect is sodium specific because high potassium or chloride concentrations did not have this effect. Under salt stress conditions, 46 different Nod factors were identified in a CIAT899 culture, compared with 29 different Nod factors under control conditions. Only 15 Nod factor structures were common to both conditions. Under salt stress conditions, 14 different new Nod factor structures were identified that were not observed as being produced under neutral or acid conditions. The implications of our results are that stress has a great influence on Nod factor biosynthesis and that new, very interesting regulatory mechanisms, worth investigating, are involved in controlling Nod factor biosynthesis
|Cite||Estévez Amador, J., Soria Díaz, M.E., Córdoba, F.F.d., Morón, B., Manyani, H., Gil Serrano, A.M.,...,Megías, M. (2009). Different and new Nod factors produced by Rhizobium tropici CIAT899 following Na+ stress. FEMS Microbiology Letters, 293 (2), 220-231.|