Show simple item record

Article

dc.creatorSoria Lozano, P.es
dc.creatorGilaberte, Y.es
dc.creatorPaz Cristobal, M. P.es
dc.creatorGarcía Luque, Isabeles
dc.date.accessioned2018-06-07T12:12:22Z
dc.date.available2018-06-07T12:12:22Z
dc.date.issued2015
dc.identifier.citationSoria Lozano, P., Gilaberte, Y., Paz Cristobal, M. y García Luque, I. (2015). In vitro effect photodynamic therapy with differents photosensitizers on cariogenic microorganisms. BMC Microbiology, 15 (187), 1-7.
dc.identifier.issn1471-2180es
dc.identifier.urihttps://hdl.handle.net/11441/75838
dc.description.abstractBackground Antimicrobial photodynamic therapy has been proposed as an alternative to suppress subgingival species. This results from the balance among Streptococcus sanguis, Streptococcus mutans and Candida albicans in the dental biofilm. Not all the photosensitizers have the same photodynamic effect against the different microorganims. The objective of this study is to compare in vitro the photodynamic effect of methylene blue (MB), rose Bengal (RB) and curcumin (CUR) in combination with white light on the cariogenic microorganism S. mutans, S. sanguis and C. albicans. Go to: Results Photodynamic therapy with MB, RB and CUR inhibited 6 log 10 the growth of both bacteria but at different concentrations: 0.31–0.62 μg/ml and 0.62–1.25 μg/ml RB were needed to photoinactivate S. mutans and S. sanguis, respectively; 1.25–2.5 μg/ml MB for both species; whereas higher CUR concentrations (80–160 μg/ml and 160–320 μg/ml) were required to obtain the same reduction in S. mutans and S. sanguis viability respectively. The minimal fungicidal concentration of MB for 5 log10 CFU reduction (4.5 McFarland) was 80–160 μg/ml, whereas for RB it ranged between 320 and 640 μg/ml. For CUR, even the maximum studied concentration (1280 μg/ml) did not reach that inhibition. Incubation time had no effect in all experiments. Go to: Conclusions Photodynamic therapy with RB, MB and CUR and white light is effective in killing S. mutans and S. sanguis strains, although MB and RB are more efficient than CUR. C. albicans required higher concentrations of all photosensitizers to obtain a fungicidal effect, being MB the most efficient and CUR ineffective.es
dc.description.sponsorshipEspaña, Ministerio de Ciencia e Innovación CTQ2013-48767-C3-2-Res
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherBioMed Centrales
dc.relation.ispartofBMC Microbiology, 15 (187), 1-7.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleIn vitro effect photodynamic therapy with differents photosensitizers on cariogenic microorganismses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.projectIDCTQ2013-48767-C3-2-Res
dc.relation.publisherversionhttp://dx.doi.org/10.1186/s12866-015-0524-3es
dc.identifier.doi10.1186/s12866-015-0524-3es
idus.format.extent7 p.es
dc.journaltitleBMC Microbiologyes
dc.publication.volumen15es
dc.publication.issue187es
dc.publication.initialPage1es
dc.publication.endPage7es
dc.contributor.funderMinisterio de Ciencia e Innovación (MICIN). España

FilesSizeFormatViewDescription
pubmed12866_2015_Article_524.pdf1.159MbIcon   [PDF] View/Open  

This item appears in the following collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional