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dc.creatorPinto Pérez, Francisco
dc.creatorPintado, C. Oscares
dc.creatorPennefather, Jocelyn
dc.creatorPatak, Evaes
dc.creatorCandenas de Luján, María Luzes
dc.identifier.citationPinto Pérez, F.M., Pintado, C.O., Pennefather, J.N., Patak, E. y Candenas de Luján, M.L. (2009). Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus. Reproductive Biology and Endocrinology, 7 (77)
dc.description.abstractBackground: In the mouse uterus, pregnancy is accompanied by changes in tachykinin and tachykinin receptor gene expression and in the uterotonic effects of endogenous tachykinins. In this study we have investigated whether changes in tachykinin expression and responses are a result of changes in ovarian steroid levels. Methods: We quantified the mRNAs of tachykinins and tachykinin receptors in uteri from ovariectomized mice and studied their regulation in response to estrogen and progesterone using real-time quantitative RT-PCR. Early (3 h) and late (24 h) responses to estrogen were evaluated and the participation of the estrogen receptors (ER), ERalpha and ERbeta, was analyzed by treating mice with propylpyrazole triol, a selective ERalpha agonist, or diarylpropionitrile, a selective agonist of ERbeta. Results: All genes encoding tachykinins (Tac1, Tac2 and Tac4) and tachykinin receptors (Tacr1, Tacr2 and Tacr3) were expressed in uteri from ovariectomized mice. Estrogen increased Tac1 and Tacr1 mRNA after 3 h and decreased Tac1 and Tac4 expression after 24 h. Tac2 and Tacr3 mRNA levels were decreased by estrogen at both 3 and 24 h. Most effects of estrogen were also observed in animals treated with propylpyrazole triol. Progesterone treatment increased the levels of Tac2. Conclusion: These results show that the expression of tachykinins and their receptors in the mouse uterus is tightly and differentially regulated by ovarian steroids. Estrogen effects are mainly mediated by ERalpha supporting an essential role for this estrogen receptor in the regulation of the tachykinergic system in the mouse
dc.description.sponsorshipMinisterio de Educación y Ciencia BFU2005-04495-C02-01/BFI, CTQ2007-61024/BQUes
dc.description.sponsorshipJunta de Andalucía FQM261es
dc.publisherBioMed Centrales
dc.relation.ispartofReproductive Biology and Endocrinology, 7 (77)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.subjectOvarian steroidses
dc.titleOvarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uteruses
dc.journaltitleReproductive Biology and Endocrinologyes
dc.contributor.funderMinisterio de Educación y Ciencia (MEC). España
dc.contributor.funderJunta de Andalucía

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional