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dc.creatorGermani, Antoniaes
dc.creatorRomero Portillo, Franciscoes
dc.creatorHoulard, Martines
dc.creatorCamonis, Jacques H.es
dc.creatorGisselbrecht, Sylviees
dc.creatorFischer, Siegmundes
dc.creatorVarin-Blank, Nadinees
dc.date.accessioned2018-03-06T15:16:03Z
dc.date.available2018-03-06T15:16:03Z
dc.date.issued1999
dc.identifier.citationGermani, A., Romero Portillo, F., Houlard, M., Camonis, J.H., Gisselbrecht, S., Fischer, S. y Varin-Blank, N. (1999). hSiah2 is a new vav binding protein which inhibits Vav-mediated signaling pathways. Molecular and Cellular Biology, 19 (5), 3798-3807.
dc.identifier.issn0270-7306es
dc.identifier.urihttps://hdl.handle.net/11441/70837
dc.description.abstractThe hematopoietic proto-oncogene vav has been characterized as a Rac1- GDP/GTP exchanger protein which regulates cytoskeletal reorganization as well as signaling pathways leading to the activation of stress-activated protein kinases (SAPK/JNKs). Furthermore, vav overexpression enhances basal and T- cell receptor (TCR)-mediated stimulation of the nuclear factor of activated T cells (NFAT). We report here the interaction between Vav and hSiah2, a mammalian homolog of Drosophila Seven in absentia (Sina) that has been implicated in R7 photoreceptor cell formation during Drosophila eye development via the proteasome degradation pathway. Vav and hSiah2 interact in vitro and in vivo and colocalize in the cytoplasm of hematopoietic cells. The Src homology domain of Vav and the C-terminal region of hSiah2 are required for this interaction. We provide evidence for a negative regulation by hSiah2 of Vav-induced basal and TCR-mediated NFAT-dependent transcription. Overexpression of hSiah2 also inhibits the onco-Vav-induced JNK activation. Although the Vav-interacting domain is located in the C-terminal portion of hSiah2, the N-terminal region of hSiah2 is necessary for the inhibitory role that seems to be independent of the proteasome degradation.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.relation.ispartofMolecular and Cellular Biology, 19 (5), 3798-3807.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titlehSiah2 is a new vav binding protein which inhibits Vav-mediated signaling pathwayses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.publisherversionhttp://dx.doi.org/10.1128/MCB.19.5.3798es
dc.identifier.doi10.1128/MCB.19.5.3798es
idus.format.extent10es
dc.journaltitleMolecular and Cellular Biologyes
dc.publication.volumen19es
dc.publication.issue5es
dc.publication.initialPage3798es
dc.publication.endPage3807es

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Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional