Artículo
A core of three amino acids at the carboxyl-terminal region of glutamine synthetase defines its regulation in cyanobacteria
Autor/es | Saelices Gómez, Lorena
Robles Rengel, Rocio Florencio Bellido, Francisco Javier |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2015 |
Fecha de depósito | 2018-01-18 |
Publicado en |
|
Resumen | Glutamine synthetase (GS) type I is a key enzyme in nitrogen metabolism, and its activity is finely controlled by cellular carbon/nitrogen balance. In cyanobacteria, a reversible process that involves protein-protein ... Glutamine synthetase (GS) type I is a key enzyme in nitrogen metabolism, and its activity is finely controlled by cellular carbon/nitrogen balance. In cyanobacteria, a reversible process that involves protein-protein interaction with two proteins, the inactivating factors IF7 and IF17, regulates GS. Previously, we showed that three arginine residues of IFs are critical for binding and inhibition of GS. In this work, taking advantage of the specificity of GS/IFs interaction in the model cyanobacteria Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120, we have constructed a different chimeric GSs from these two cyanobacteria. Analysis of these proteins, together with a site-directed mutagenesis approach, indicates that a core of three residues (E419, N456 and R459) is essential for the inactivation process. The three residues belong to the last 56 amino acids of the C-terminus of SynechocystisGS. A protein-protein docking modeling of SynechocystisGS in complex with IF7 supports the role of the identified core for GS/IF interaction. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España Junta de Andalucía |
Identificador del proyecto | BFU 2010 - 15708
BFU 2013 - 41712 Bio - 284 |
Cita | Saelices Gómez, L., Robles Rengel, R. y Florencio Bellido, F.J. (2015). A core of three amino acids at the carboxyl-terminal region of glutamine synthetase defines its regulation in cyanobacteria. Molecular Microbiology, 96, 483-496. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
csic9Saelices et. al. revised.pdf | 522.0Kb | [PDF] | Ver/ | |