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dc.creatorMariscal Romero, Vicentees
dc.creatorHerrero Moreno, Antoniaes
dc.creatorFlores García, Enriquees
dc.date.accessioned2018-01-09T17:29:06Z
dc.date.available2018-01-09T17:29:06Z
dc.date.issued2016
dc.identifier.citationMariscal Romero, V., Herrero Moreno, A. y Flores García, E. (2016). Overexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaena. Molecular Microbiology, 101 (6), 968-981.
dc.identifier.issn0950-382Xes
dc.identifier.urihttp://hdl.handle.net/11441/68543
dc.description.abstractFilamentous, N2 -fixing, heterocyst-forming cyanobacteria grow as chains of cells that are connected by septal junctions. In the model organism Anabaena sp. strain PCC 7120, the septal protein SepJ is required for filament integrity, normal intercellular molecular exchange, heterocyst differentiation, and diazotrophic growth. An Anabaena strain overexpressing SepJ made wider septa between vegetative cells than the wild type, which correlated with a more spread location of SepJ in the septa as observed with a SepJ-GFP fusion, and contained an increased number of nanopores, the septal peptidoglycan perforations that likely accommodate septal junctions. The septa between heterocysts and vegetative cells, which are narrow in wild-type Anabaena, were notably enlarged in the SepJ-overexpressing mutant. Intercellular molecular exchange tested with fluorescent tracers was increased for the SepJ-overexpressing strain specifically in the case of calcein transfer between vegetative cells and heterocysts. These results support an association between calcein transfer, SepJ-related septal junctions, and septal peptidoglycan nanopores. Under nitrogen deprivation, the SepJ-overexpressing strain produced an increased number of contiguous heterocysts but a decreased percentage of total heterocysts. These effects were lost or altered in patS and hetN mutant backgrounds, supporting a role of SepJ in the intercellular transfer of regulatory signals for heterocyst differentiationes
dc.description.sponsorshipEspaña, Plan nacional de Investigación BFU2014-56757-Pes
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherWileyes
dc.relation.ispartofMolecular Microbiology, 101 (6), 968-981.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBacterial developmentes
dc.subjectCyanobacteriaes
dc.subjectHeterocyst differentiationes
dc.subjectIntercellular communicationes
dc.titleOverexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaenaes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/submittedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDBFU2014-56757-Pes
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/mmi.13436/epdfes
dc.identifier.doi10.1111/mmi.13436.es
idus.format.extent15 p.es
dc.journaltitleMolecular Microbiologyes
dc.publication.volumen101es
dc.publication.issue6es
dc.publication.initialPage968es
dc.publication.endPage981es

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