dc.creator | Alonso, Concepción | es |
dc.creator | Balao Robles, Francisco J. | es |
dc.creator | Bazaga, Pilar | es |
dc.creator | Pérez, Ricardo | es |
dc.date.accessioned | 2018-01-04T11:37:08Z | |
dc.date.available | 2018-01-04T11:37:08Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Alonso, C., Balao Robles, F.J., Bazaga, P. y Pérez, R. (2016). Epigenetic contribution to successful polyploidizations: variation in global cytosine methylation along an extensive ploidy series in Dianthus broteri (Caryophyllaceae). New Phytologist, 2016 (212), 571-576. | |
dc.identifier.issn | 1469-8137 | es |
dc.identifier.uri | http://hdl.handle.net/11441/68271 | |
dc.description.abstract | Polyploidization is a significant evolutionary force in plants which involves major
genomic and genetic changes, frequently regulated by epigenetic factors. We
explored whether natural polyploidization in Dianthus broteri complex resulted in
substantial changes in global DNA cytosine methylation associated to ploidy.
Global cytosine methylation was estimated by HPLC in 12 monocytotypic
populations with different ploidies (2x, 4x, 6x, 12x) broadly distributed within D.
broteri distribution range. The effects of ploidy level and local variation on
methylation were assessed by GLMM.
Dianthus broteri exhibited a higher methylation percent (~33%) than expected by its
monoploid genome size and a large variation among study populations (range: 29.3
- 35.3%). Global methylation tended to increase with ploidy but did not significantly
differ across levels due to increased variation within the highest-order polyploidy
categories. Methylation varied more among hexaploid and dodecaploid populations,
despite such cytotypes show more restricted geographic location and increased
genetic relatedness than diploids and tetraploids.
In this study, we demonstrate the usefulness of an HPLC method in providing
precise and genome reference-free global measure of DNA cytosine methylation,
suitable to advance current knowledge of the roles of this epigenetic mechanism in
polyploidization processes. | es |
dc.description.sponsorship | MINECO SEV-2012-0262 | es |
dc.description.sponsorship | CGL2013-43352-P | es |
dc.description.sponsorship | CGL2013-45463-P | es |
dc.description.sponsorship | Junta de Andalucía 34/2004 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Blackwell Publishing | es |
dc.relation.ispartof | New Phytologist, 2016 (212), 571-576. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | autopolyploidy | es |
dc.subject | DNA methylation | es |
dc.subject | endemism | es |
dc.subject | genome size | es |
dc.subject | genomic shock | es |
dc.title | Epigenetic contribution to successful polyploidizations: variation in global cytosine methylation along an extensive ploidy series in Dianthus broteri (Caryophyllaceae) | es |
dc.title.alternative | Alonso, Conchita ; Balao, Francisco; Bazaga, Pilar ; Pérez, Ricardo | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Vegetal y Ecología | es |
dc.relation.projectID | SEV-2012-0262 | es |
dc.relation.projectID | 34/2004 | es |
dc.relation.projectID | CGL2013-43352-P | es |
dc.relation.projectID | CGL2013-45463-P | es |
dc.relation.publisherversion | http://nph.onlinelibrary.wiley.com/hub/journal/10.1111/(ISSN)1469-8137/ | es |
dc.identifier.doi | 10.1111/nph.14138 | es |
dc.contributor.group | Universidad de Sevilla. RNM204: Ecologia Reproductiva de Plantas | es |
idus.format.extent | 6 p. | es |
dc.journaltitle | New Phytologist | es |
dc.publication.volumen | 2016 | es |
dc.publication.issue | 212 | es |
dc.publication.initialPage | 571 | es |
dc.publication.endPage | 576 | es |
dc.identifier.sisius | 11397 | es |