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dc.creatorTorres Ruiz, Blases
dc.creatorBeltrán Matas, Pabloes
dc.creatorLuque Laó, María Ángeleses
dc.creatorHerrero Rama, Luis Jacintoes
dc.date.accessioned2017-11-09T14:28:21Z
dc.date.available2017-11-09T14:28:21Z
dc.date.issued2017
dc.identifier.citationTorres Ruiz, B., Beltrán Matas, P., Luque Laó, M.Á. y Herrero Rama, L.J. (2017). Excitability is increased in hippocampal CA1 pyramidal cells of Fmr1 knockout mice. Plos One, 12 (9), 1-18.
dc.identifier.issn1932-6203es
dc.identifier.urihttp://hdl.handle.net/11441/65870
dc.description.abstractFragile X syndrome (FXS) is caused by a failure of neuronal cells to express the gene encoding the fragile mental retardation protein (FMRP). Clinical features of the syndrome include intellectual disability, learning impairment, hyperactivity, seizures and anxiety. Fmr1 knockout (KO) mice do not express FMRP and, as a result, reproduce some FXS behavioral abnormalities. While intrinsic and synaptic properties of excitatory cells in various part of the brain have been studied in Fmr1 KO mice, a thorough analysis of action potential characteristics and input-output function of CA1 pyramidal cells in this model is lacking. With a view to determining the effects of the absence of FMRP on cell excitability, we studied rheobase, action potential duration, firing frequency-current intensity relationship and action potential after-hyperpolarization (AHP) in CA1 pyramidal cells of the hippocampus of wild type (WT) and Fmr1 KO male mice. Brain slices were prepared from 8- to 12-week-old mice and the electrophysiological properties of cells recorded. Cells from both groups had similar resting membrane potentials. In the absence of FMRP expression, cells had a significantly higher input resistance, while voltage threshold and depolarization voltage were similar in WT and Fmr1 KO cell groups. No changes were observed in rheobase. The action potential duration was longer in the Fmr1 KO cell group, and the action potential firing frequency evoked by current steps of the same intensity was higher. Moreover, the gain (slope) of the relationship between firing frequency and injected current was 1.25-fold higher in the Fmr1 KO cell group. Finally, AHP amplitude was significantly reduced in the Fmr1 KO cell group. According to these data, FMRP absence increases excitability in hippocampal CA1 pyramidal cells.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPlos One, 12 (9), 1-18.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAction Potentialses
dc.subjectfragile X mental retardation proteines
dc.subjectPyramidal Cellses
dc.titleExcitability is increased in hippocampal CA1 pyramidal cells of Fmr1 knockout micees
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Fisiologíaes
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0185067es
dc.identifier.doi10.1371/journal.pone.0185067es
idus.format.extent19 p.es
dc.journaltitlePlos Onees
dc.publication.volumen12es
dc.publication.issue9es
dc.publication.initialPage1es
dc.publication.endPage18es

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Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional