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dc.creatorGarcía Luque, Isabeles
dc.creatorPascual Hernández, Álvaroes
dc.creatorGuzman Morgaz, Carmenes
dc.creatorPerea Pérez, Evelio Josées
dc.date.accessioned2017-10-04T16:49:40Z
dc.date.available2017-10-04T16:49:40Z
dc.date.issued1992-01-01
dc.identifier.citationGarcía Luque, I., Pascual Hernández, Á., Guzman Morgaz, C. y Perea Pérez, E.J. (1992). Uptake and intracellular activity of sparfloxacin in human polymorphonuclear leukocytes and tissue culture cells. Antimicrobial Agents and Chemotherapy, 36 (5), 1053-1056.
dc.identifier.issn0066-4804 (impreso)es
dc.identifier.issn1098-6596 (electrónico)es
dc.identifier.urihttp://hdl.handle.net/11441/65007
dc.description.abstractThe penetration of sparfloxacin into human neutrophils (PMN) and different tissue culture cells (HEp-2 and McCoy) was evaluated. The cellular to extracellular concentration ratios (C/E) of sparfloxacin were always higher than 4 at extracellular concentrations ranging from 0.5 to 25 mg/liter. The uptake of sparfloxacin by PMN was rapid, nonsaturable, reversible, not energy dependent, and significantly reduced at pH 8. The penetration of this agent into PMN was similar when viable and Formalin-killed cells were used and was not affected by environmental temperature. Ingestion of opsonized zymosan significantly increased the amount of PMN-associated sparfloxacin. Sparfloxacin at a concentration of 0.5 mg induced a significant reduction in the survival of intracellular Staphylococcus aureus. It is concluded that sparfloxacin reaches intracellular concentrations within leukocytic cells much higher than extracellular concentrations, while remaining active intracellularly.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.relation.ispartofAntimicrobial Agents and Chemotherapy, 36 (5), 1053-1056.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleUptake and intracellular activity of sparfloxacin in human polymorphonuclear leukocytes and tissue culture cellses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
idus.format.extent4 p.es
dc.journaltitleAntimicrobial Agents and Chemotherapyes
dc.publication.volumen36es
dc.publication.issue5es
dc.publication.initialPage1053es
dc.publication.endPage1056es

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