Management of enzyme diversity in high-performance cellulolytic cocktails
|Author||Reyes Sosa, Francisco Manuel
López Morales, Macarena
Platero Gómez, Ana Isabel
Valbuena Crespo, Noelia
Sánchez Zamorano, Laura
Rocha Martín, Javier
Molina Heredia, Fernando Publio
|Department||Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular|
|Published in||Biotechnology for Biofuels, 10 (156), 1-10.|
Modern biorefineries require enzymatic cocktails of improved efficiency to generate fermentable sugars from lignocellulosic biomass. Cellulolytic fungi, among other microorganisms, have demonstrated the highest ...
Background Modern biorefineries require enzymatic cocktails of improved efficiency to generate fermentable sugars from lignocellulosic biomass. Cellulolytic fungi, among other microorganisms, have demonstrated the highest potential in terms of enzymatic productivity, complexity and efficiency. On the other hand, under cellulolytic-inducing conditions, they often produce a considerable diversity of carbohydrate-active enzymes which allow them to adapt to changing environmental conditions. However, industrial conditions are fixed and adjusted to the optimum of the whole cocktail, resulting in underperformance of individual enzymes. Results One of these cellulolytic cocktails from Myceliophthora thermophila has been analyzed here by means of LC–MS/MS. Pure GH6 family members detected have been characterized, confirming previous studies, and added to whole cocktails to compare their contribution in the hydrolysis of industrial substrates. Finally, independent deletions of two GH6 family members, as an example of the enzymatic diversity management, led to the development of a strain producing a more efficient cellulolytic cocktail. Conclusions These data indicate that the deletion of noncontributive cellulases (here EG VI) can increase the cellulolytic efficiency of the cocktail, validating the management of cellulase diversity as a strategy to obtain improved fungal cellulolytic cocktails.
|Citation||Reyes Sosa, F.M., López Morales, M., Platero Gómez, A.I., Valbuena Crespo, N., Sánchez Zamorano, L., Rocha Martín, J. y Molina Heredia, F.P. (2017). Management of enzyme diversity in high-performance cellulolytic cocktails. Biotechnology for Biofuels, 10 (156), 1-10.|