dc.creator | Petersen, Eskild | es |
dc.creator | Borobio Enciso, María Victoria | es |
dc.creator | Guy, Edward | es |
dc.creator | Liesenfeld, Oliver | es |
dc.creator | Meroni, Valeria | es |
dc.creator | Naessens, Anne | es |
dc.creator | Spranzi, Emma | es |
dc.creator | Thulliez, Philippe | es |
dc.date.accessioned | 2017-07-27T11:39:08Z | |
dc.date.available | 2017-07-27T11:39:08Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | Petersen, E., Borobio Enciso, M.V., Guy, E., Liesenfeld, O., Meroni, V., Naessens, A.,...,Thulliez, P. (2005). European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index. Journal of Clinical Microbiology, 43 (4), 1570-1574. | |
dc.identifier.issn | 0095-1137 (impreso) | es |
dc.identifier.issn | 1098-660X (electronico) | es |
dc.identifier.uri | http://hdl.handle.net/11441/63340 | |
dc.description.abstract | The LIAISON system is a fully automated system based on chemiluminescence and antigen bound to magnetic microparticles. The system allows fast and precise measurement of Toxoplasma-specific immunoglobulin G (IgG) and IgM antibody levels and measurement of the IgG avidity index even at low levels of Toxoplasma-specific IgG antibodies in a single step without manual interference. Seven European centers participated in a multicenter evaluation of the LIAISON system. The sensitivity and specificity of the LIAISON system compared to the Sabin-Feldman dye test were 99.3 and 96.8%, respectively. In a comparison of the LIAISON Toxoplasma-specific IgM assay with an immunosorbent agglutination assay, the LIAISON assay had a sensitivity of 96.7% and a specificity of 95.4%. The LIAISON IgG assay showed agreements of 91, 100, and 100% with the AXSYM IgG (Abbott), VIDAS IgG (bioMérieux), and Platelia IgG (Bio-Rad) assays, respectively. The LIAISON IgM assay showed agreements of 95% with the AXSYM IgM and Platelia IgM assays, 96% with the ISAGA IgM assay (bioMérieux), and 97% with the VIDAS IgM assay. The coefficient of correlation between the LIAISON system and the VIDAS Toxoplasma-specific IgG avidity index was 0.81. By use of the Toxoplasma-specific IgG avidity index assay with specific IgM-positive samples, the diagnosis of infection with Toxoplasma gondii in early pregnancy has been improved significantly. The LIAISON avidity assay is a valuable assay for the exclusion of recently acquired infection with T. gondii (less than 4 months) in pregnant women, and it decreases significantly the necessity for follow-up testing. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Journal of Clinical Microbiology, 43 (4), 1570-1574. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/JCM.43.4.1570-1574.2005 | es |
dc.identifier.doi | 10.1128/JCM.43.4.1570-1574.2005 | es |
idus.format.extent | 5 p. | es |
dc.journaltitle | Journal of Clinical Microbiology | es |
dc.publication.volumen | 43 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 1570 | es |
dc.publication.endPage | 1574 | es |