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Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: Purification and characterization of the enzyme
dc.creator | Peciña, Ana | es |
dc.creator | Pascual, Alberto | es |
dc.creator | Paneque Guerrero, Antonio | es |
dc.date.accessioned | 2017-07-25T11:12:33Z | |
dc.date.available | 2017-07-25T11:12:33Z | |
dc.date.issued | 1999 | |
dc.identifier.citation | Peciña, A., Pascual, A. y Paneque, A. (1999). Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: Purification and characterization of the enzyme. Journal of Bacteriology, 181 (5), 1409-1414. | |
dc.identifier.issn | 0021-9193 (impreso) | es |
dc.identifier.issn | 1098-5530 (electronico) | es |
dc.identifier.uri | http://hdl.handle.net/11441/63096 | |
dc.description.abstract | The alginate lyase-encoding gene (algL) of Azotobacter chroococcum was localized to a 3.1-kb EcoRI DNA fragment that revealed an open reading frame of 1,116 bp. This open reading frame encodes a protein of 42.98 kDa, in agreement with the value previously reported by us for this protein. The deduced protein has a potential N-terminal signal peptide that is consistent with its proposed periplasmic location. The analysis of the deduced amino acid sequence indicated that the gene sequence has a high homology (90% identity) to the Azotobacter vinelandii gene sequence, which has very recently been deposited in the GenBank database, and that it has 64% identity to the Pseudomonas aeruginosa gene sequence but that it has rather low homology (15 to 22% identity) to the gene sequences encoding alginate lyase in other bacteria. The A. chroococcum AlgL protein was overproduced in Escherichia coli and purified to electrophoretic homogeneity in a two-step chromatography procedure on hydroxyapatite and phenyl-Sepharose. The kinetic and molecular parameters of the recombinant alginate lyase are similar to those found for the native enzyme. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Journal of Bacteriology, 181 (5), 1409-1414. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: Purification and characterization of the enzyme | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
idus.format.extent | 6 p. | es |
dc.journaltitle | Journal of Bacteriology | es |
dc.publication.volumen | 181 | es |
dc.publication.issue | 5 | es |
dc.publication.initialPage | 1409 | es |
dc.publication.endPage | 1414 | es |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Cloning and Expression.pdf | 1.141Mb | [PDF] | Ver/ | |