dc.creator | García Domínguez, Mario | es |
dc.creator | López Maury, Luis | es |
dc.creator | Florencio Bellido, Francisco Javier | es |
dc.creator | Reyes Rosa, José Carlos | es |
dc.date.accessioned | 2017-07-25T11:10:49Z | |
dc.date.available | 2017-07-25T11:10:49Z | |
dc.date.issued | 2000 | |
dc.identifier.citation | García Domínguez, M., López Maury, L., Florencio Bellido, F.J. y Reyes Rosa, J.C. (2000). A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803. Journal of Bacteriology, 182 (6), 1507-1514. | |
dc.identifier.issn | 0021-9193 (impreso) | es |
dc.identifier.issn | 1098-5530 (electronico) | es |
dc.identifier.uri | http://hdl.handle.net/11441/63095 | |
dc.description.abstract | A gene cluster composed of nine open reading frames (ORFs) involved in Ni2+, Co2+, and Zn2+ sensing and tolerance in the cyanobacterium Synechocystis sp. Strain PCC 6803 has been identified. The cluster includes an Ni2+ response operon and a Co2+ response system, as well as a Zn2+ response system previously described. Expression of the Ni2+ response operon (nrs) was induced in the presence of Ni2+ and Co2+. Reduced Ni2+ tolerance was observed following disruption of two ORFs of the operon (nrsA and nrsD). We also show that the nrsD gene encodes a putative Ni2+ permease whose carboxy-terminal region is a metal binding domain. The Co2+ response system is composed of two divergently transcribed genes, corR and corT, mutants of which showed decreased Co2+ tolerance. Additionally, corR mutants showed an absence of Co2+-dependent induction of corT, indicating that CorR is a transcriptional activator of corT. To our knowledge, CorR is the first Co2+-sensing transcription factor described. Our data suggest that this region of the Synechocystis sp. strain PCC 6803 genome is involved in sensing and homeostasis of Ni2+, Co2+, and Zn2+. | es |
dc.description.sponsorship | Ministerio de Educación y Cultura PB97-0732 | es |
dc.description.sponsorship | Junta de Andalucía CV1-0112 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Journal of Bacteriology, 182 (6), 1507-1514. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | PB97-0732 | es |
dc.relation.projectID | CV1-0112 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/JB.182.6.1507-1514.2000 | es |
dc.identifier.doi | 10.1128/JB.182.6.1507-1514.2000 | es |
idus.format.extent | 8 p. | es |
dc.journaltitle | Journal of Bacteriology | es |
dc.publication.volumen | 182 | es |
dc.publication.issue | 6 | es |
dc.publication.initialPage | 1507 | es |
dc.publication.endPage | 1514 | es |
dc.contributor.funder | Ministerio de Educación y Cultura (MEC). España | |
dc.contributor.funder | Junta de Andalucía | |