dc.creator | Muro Pastor, María Isabel | es |
dc.creator | Flores García, Enrique | es |
dc.creator | Herrero Moreno, Antonia | es |
dc.date.accessioned | 2017-07-21T15:49:46Z | |
dc.date.available | 2017-07-21T15:49:46Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Muro Pastor, M.I., Flores García, E. y Herrero, A. (2009). NtcA-regulated heterocyst differentiation genes hetC and devB from Anabaena sp. strain PCC 7120 exhibit a similar tandem promoter arrangement. Journal of Bacteriology, 191 (18), 5765-5774. | |
dc.identifier.issn | 0021-9193 (impreso) | es |
dc.identifier.issn | 1098-5530 (electronico) | es |
dc.identifier.uri | http://hdl.handle.net/11441/62905 | |
dc.description.abstract | Transcription of the hetC gene, whose product is required for heterocyst differentiation, takes place from a long promoter region that includes the previously described HetR-independent, NtcA-activated promoter producing transcripts with a 5′ end corresponding to position -571 with respect to the translational start site of hetC. Northern blot analysis indicated that the accumulation of hetC transcripts depends on HetR, and a second transcriptional start site located at position -293 that leads to NtcA-dependent, HetR-dependent inducible transcription of hetC was identified. Upon nitrogen stepdown, expression of a PhetC::gfp fusion was transiently induced in specific cells that were differentiating into heterocysts, both when the whole promoter region (containing transcription start points -571 and -293) or a short version (containing only the transcription start point -293) was used. Expression of hetC from the -293 position was delayed in a strain bearing a deleted promoter region lacking sequences upstream from position -570. Such a strain was still able to differentiate functional heterocysts and to grow diazotrophically, although diazotrophic growth was impaired under certain conditions. Similarly, a second, NtcA-dependent, HetR-dependent transcriptional start site was identified at position -454 in the promoter region upstream from the devBCA operon encoding an ABC transport system involved in heterocyst maturation, in which an NtcA-dependent promoter producing transcripts starting at position -704 had been previously noted. Thus, the hetC and devBCA promoter regions exhibit similar tandem promoter arrangements. | es |
dc.description.sponsorship | Ministerio de Educación y Ciencia BFU2007-60457 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Journal of Bacteriology, 191 (18), 5765-5774. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | NtcA-regulated heterocyst differentiation genes hetC and devB from Anabaena sp. strain PCC 7120 exhibit a similar tandem promoter arrangement | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/JB.00616-09 | es |
dc.identifier.doi | 10.1128/JB.00616-09 | es |
idus.format.extent | 10 p. | es |
dc.journaltitle | Journal of Bacteriology | es |
dc.publication.volumen | 191 | es |
dc.publication.issue | 18 | es |
dc.publication.initialPage | 5765 | es |
dc.publication.endPage | 5774 | es |
dc.contributor.funder | Ministerio de Educación y Ciencia (MEC). España | |