Mostrar el registro sencillo del ítem

Artículo

dc.creatorPuerta Fernández, Elenaes
dc.creatorVioque Peña, Agustínes
dc.date.accessioned2017-07-20T16:02:34Z
dc.date.available2017-07-20T16:02:34Z
dc.date.issued2011
dc.identifier.citationPuerta Fernández, E. y Vioque Peña, A. (2011). Hfq Is Required for Optimal Nitrate Assimilation in the Cyanobacterium Anabaena sp. Strain PCC 7120. Journal of Bacteriology, 193 (14), 3546-3555.
dc.identifier.issn0021-9193 (impreso)es
dc.identifier.issn1098-5530 (electronico)es
dc.identifier.urihttp://hdl.handle.net/11441/62804
dc.description.abstractHfq is an RNA binding protein involved in posttranscriptional regulation of gene expression in bacteria. It acts by binding to regulatory small RNAs (sRNAs), which confer specificity for the regulation. Recently, orthologues of the Hfq protein were annotated in cyanobacterial genomes, although its capacity to regulate gene expression by interacting with sRNAs has not been yet demonstrated. Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium that, in the absence of combined nitrogen, is able to fix atmospheric nitrogen by differentiating specialized cells called heterocysts. We have generated an hfq knockout mutant of Anabaena sp. PCC 7120. Deletion of this gene results in differentiation of heterocysts in the presence of nitrate, suggesting a defect in nitrate assimilation. We show that hfq mutant cells are affected in transport and use of nitrate and nitrite. An analysis of the expression of several genes in the nir operon, encoding different elements of the nitrate assimilation pathway, demonstrates a downregulation of their transcription in mutant cells. We also observed that genes ntcB and cnaT, involved in the regulation of the nir operon, show a lower expression in cells lacking Hfq. Finally, when hfq was reintroduced in the mutant, heterocyst differentiation was no longer observed in the presence of nitrate. Therefore, our results indicate that the RNA chaperone Hfq is involved in the regulation of the nir operon, although the mechanism for this regulation is still unknown.es
dc.description.sponsorshipMinisterio de Ciencia e Innovación y European Regional Fund BFU2007-60651 BFU2010-14821es
dc.description.sponsorshipPlan Andaluz de Investigación BIO215es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.relation.ispartofJournal of Bacteriology, 193 (14), 3546-3555.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleHfq Is Required for Optimal Nitrate Assimilation in the Cyanobacterium Anabaena sp. Strain PCC 7120es
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDBFU2007-60651es
dc.relation.projectIDBFU2010-14821es
dc.relation.projectIDBIO215es
dc.relation.publisherversionhttp://dx.doi.org/10.1128/JB.00254-11es
dc.identifier.doi10.1128/JB.00254-11es
idus.format.extent10 p.es
dc.journaltitleJournal of Bacteriologyes
dc.publication.volumen193es
dc.publication.issue14es
dc.publication.initialPage3546es
dc.publication.endPage3555es
dc.contributor.funderMinisterio de Ciencia e Innovación (MICIN). España
dc.contributor.funderJunta de Andalucía

FicherosTamañoFormatoVerDescripción
Hfq Is Required.pdf1.164MbIcon   [PDF] Ver/Abrir  

Este registro aparece en las siguientes colecciones

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como: Attribution-NonCommercial-NoDerivatives 4.0 Internacional