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dc.creatorRallis, Charalamposes
dc.creatorLópez Maury, Luises
dc.creatorGeorgescu, Teodoraes
dc.creatorPancaldi, Veraes
dc.creatorBähler, Jürg Ürges
dc.date.accessioned2017-04-11T11:50:11Z
dc.date.available2017-04-11T11:50:11Z
dc.date.issued2014
dc.identifier.issn2046-6390es
dc.identifier.urihttp://hdl.handle.net/11441/57475
dc.description.abstractTarget of rapamycin complex 1 (TORC1), which controls growth in response to nutrients, promotes ageing in multiple organisms. The fission yeast Schizosaccharomyces pombe emerges as a valuable genetic model system to study TORC1 function and cellular ageing. Here we exploited the combinatorial action of rapamycin and caffeine, which inhibit fission yeast growth in a TORC1-dependent manner. We screened a deletion library, comprising ∼84% of all non-essential fission yeast genes, for drug-resistant mutants. This screen identified 33 genes encoding functions such as transcription, kinases, mitochondrial respiration, biosynthesis, intra-cellular trafficking, and stress response. Among the corresponding mutants, 5 showed shortened and 21 showed increased maximal chronological lifespans; 15 of the latter mutants showed no further lifespan increase with rapamycin and might thus represent key targets downstream of TORC1. We pursued the long-lived sck2 mutant with additional functional analyses, revealing that the Sck2p kinase functions within the TORC1 network and is required for normal cell growth, global protein translation, and ribosomal S6 protein phosphorylation in a nutrient-dependent manner. Notably, slow cell growth was associated with all long-lived mutants while oxidative-stress resistance was not.es
dc.description.sponsorshipBBSRC Research Grant BB/I012451/1es
dc.description.sponsorshipWellcome Trust Senior Investigator Award 095598/Z/11/Zes
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherCompany of Biologists: OAJes
dc.relation.ispartofBiology Open, 3 (2), 161-171.es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectTORes
dc.subjectHronological lifespanes
dc.subjectOxidative stresses
dc.subjectCinase Sck2es
dc.subjectProtein translationes
dc.titleSystematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growthes
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDBB/I012451/1es
dc.relation.projectID095598/Z/11/Zes
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/es
dc.relation.publisherversionhttp://dx.doi.org/10.1242/bio.20147245es
dc.identifier.doi10.1242/bio.20147245es
idus.format.extent10 p.es
dc.journaltitleBiology Openes
dc.publication.volumen3es
dc.publication.issue2es
dc.publication.initialPage161es
dc.publication.endPage171es
dc.contributor.funderWellcome Trust

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Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional