Mostrar el registro sencillo del ítem

Artículo

dc.creatorGiner Lamia, Joaquínes
dc.creatorLópez Maury, Luises
dc.creatorFlorencio Bellido, Francisco Javieres
dc.date.accessioned2017-03-17T08:51:19Z
dc.date.available2017-03-17T08:51:19Z
dc.date.issued2014
dc.identifier.citationGiner Lamia, J., López Maury, L. y Florencio Bellido, F.J. (2014). Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803. Plos One, 9 (9), e108912-1-e108912-16.
dc.identifier.issn1932-6203es
dc.identifier.urihttp://hdl.handle.net/11441/55940
dc.description.abstractCopper is an essential element involved in fundamental processes like respiration and photosynthesis. However, it becomes toxic at high concentration, which has forced organisms to control its cellular concentration. We have recently described a copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803, which is mediated by the two-component system, CopRS, a RND metal transport system, CopBAC and a protein of unknown function, CopM. Here, we report the transcriptional responses to copper additions at non-toxic (0.3 μM) and toxic concentrations (3 μM) in the wild type and in the copper sensitive copR mutant strain. While 0.3 μM copper slightly stimulated metabolism and promoted the exchange between cytochrome c6 and plastocyanin as soluble electron carriers, the addition of 3 μM copper catalyzed the formation of ROS, led to a general stress response and induced expression of Fe-S cluster biogenesis genes. According to this, a double mutant strain copRsufR, which expresses constitutively the sufBCDS ope ron, tolerated higher copper concentration than the copR mutant strain, suggesting that Fe-S clusters are direct targets of copper toxicity in Synechocystis. In addition we have also demonstrated that InrS, a nickel binding transcriptional repressor that belong to the CsoR family of transcriptional factor, was involved in heavy metal homeostasis, including copper, in Synechocystis. Finally, global gene expression analysis of the copR mutant strain suggested that CopRS only controls the expression of copMRS and copBAC operons in response to copper.es
dc.description.sponsorshipJunta de Andalucía BIO–284es
dc.description.sponsorshipMinisterio de Economía y Competitividad BFU2010– 15708es
dc.description.sponsorshipUnión Europea FEDER E–CLPN09–001es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPlos One, 9 (9), e108912-1-e108912-16.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBacterial Proteinses
dc.subjectCopperes
dc.subjectCytochromes c6es
dc.subjectDNA-Binding Proteinses
dc.subjectEnvironmental Pollutantses
dc.subjectGene Expression Profilinges
dc.subjectGene Expression Regulationes
dc.subjectMolecular Sequence Annotationes
dc.subjectPlastocyanines
dc.subjectReactive Oxygen Specieses
dc.subjectSynechocystises
dc.subjectTranscriptomees
dc.titleGlobal transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803es
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDBIO–284es
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/BFU2010–15708es
dc.relation.projectIDFEDER E–CLPN09–001es
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0108912es
dc.identifier.doi10.1371/journal.pone.0108912es
idus.format.extent16 p.es
dc.journaltitlePlos Onees
dc.publication.volumen9es
dc.publication.issue9es
dc.publication.initialPagee108912-1es
dc.publication.endPagee108912-16es

FicherosTamañoFormatoVerDescripción
Global transcriptional profiles.pdf1.123MbIcon   [PDF] Ver/Abrir  

Este registro aparece en las siguientes colecciones

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como: Attribution-NonCommercial-NoDerivatives 4.0 Internacional