dc.creator | Groth, Petra | es |
dc.creator | Orta Vázquez, Manuel Luis | es |
dc.creator | Elvers, Ingegerd | es |
dc.creator | Majumder, Muntasir Mamun | es |
dc.creator | Lagerqvist, Anne | |
dc.creator | Helleday, Thomas | |
dc.date.accessioned | 2017-03-15T13:44:44Z | |
dc.date.available | 2017-03-15T13:44:44Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Groth, P., Orta Vázquez, M.L., Elvers, I., Majumder, M.M., Lagerqvist, A. y Helleday, T. (2012). Homologous recombination repairs secondary replication induced DNA double-strand breaks after ionizing radiation. Nucleic Acids Research, 40 (14), 6585-6594. | |
dc.identifier.issn | 0305-1048 | es |
dc.identifier.uri | http://hdl.handle.net/11441/55888 | |
dc.description.abstract | Ionizing radiation (IR) produces direct two-ended DNA double-strand breaks (DSBs) primarily repaired by non-homologous end joining (NHEJ). It is, however, well established that homologous recombination (HR) is induced and required for repair of a subset of DSBs formed following IR. Here, we find that HR induced by IR is drastically reduced when post-DNA damage replication is inhibited in mammalian cells. Both IR-induced RAD51 foci and HR events in the hprt gene are reduced in the presence of replication polymerase inhibitor aphidicolin (APH). Interestingly, we also detect reduced IR-induced toxicity in HR deficient cells when inhibiting post-DNA damage replication. When studying DSB formation following IR exposure, we find that apart from the direct DSBs the treatment also triggers formation of secondary DSBs peaking at 7–9 h after exposure. These secondary DSBs are restricted to newly replicated DNA and abolished by inhibiting post-DNA damage replication. Further, we find that IR-induced RAD51 foci are decreased by APH only in cells replicating at the time of IR exposure, suggesting distinct differences between IR-induced HR in S- and G2-phases of the cell cycle. Altogether, our data indicate that secondary replication-associated DSBs formed following exposure to IR are major substrates for IR-induced HR repair. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Oxford University Press | es |
dc.relation.ispartof | Nucleic Acids Research, 40 (14), 6585-6594. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Homologous recombination repairs secondary replication induced DNA double-strand breaks after ionizing radiation | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.relation.publisherversion | 10.1093/nar/gks315 | es |
dc.identifier.doi | 10.1093/nar/gks315 | es |
idus.format.extent | 10 p. | es |
dc.journaltitle | Nucleic Acids Research | es |
dc.publication.volumen | 40 | es |
dc.publication.issue | 14 | es |
dc.publication.initialPage | 6585 | es |
dc.publication.endPage | 6594 | es |