Mostrar el registro sencillo del ítem

Artículo

dc.creatorAlbi Rodríguez, Tomáses
dc.creatorRuiz, María Teresaes
dc.creatorReyes, Pedro de loses
dc.creatorValverde Albacete, Federicoes
dc.creatorRomero Rodríguez, José Maríaes
dc.date.accessioned2017-03-14T13:11:21Z
dc.date.available2017-03-14T13:11:21Z
dc.date.issued2016
dc.identifier.citationAlbi, T., Ruiz, M.T., Reyes, P.d.l., Valverde Albacete, F. y Romero Rodríguez, J.M. (2016). Characterization of the sucrose phosphate phosphatase (SPP) isoforms from Arabidopsis thaliana and role of the S6PPc domain in dimerization. Plos One, 11 (11), e0166308-1-e0166308-19.
dc.identifier.issn1932-6203es
dc.identifier.urihttp://hdl.handle.net/11441/55839
dc.description.abstractSucrose-phosphate phosphatase (SPP) catalyses the final step in the sucrose biosynthesis pathway. Arabidopsis thaliana genome codifies four SPP isoforms. In this study, the four Arabidopsis thaliana genes coding for SPP isoforms have been cloned, expressed in Escherichia coli and the kinetic and regulatory properties of the purified enzymes analysed. SPP2 is the isoform showing the highest activity, with SPP3b and SPP3a showing lower activity levels. No activity was detected for SPP1. We propose that this lack of activity is probably due to the absence of an essential amino acid participating in catalysis and/or in the binding of the substrate, sucrose-6-phosphate (Suc6P). The expression patterns of Arabidopsis SPP genes indicate that SPP2 and SPP3b are the main isoforms expressed in different tissues and organs, although the non-catalytic SPP1 is the main isoform expressed in roots. Thus, SPP1 could have acquired new unknown functions. We also show that the three catalytically active SPPs from Arabidopsis are dimers. By generating a chimeric SPP composed of the monomeric cyanobacterial SPP fused to the higher plant non-catalytic S6PPc domain (from SPP2), we show that the S6PPc domain is responsible for SPP dimerization. This is the first experimental study on the functionality and gene expression pattern of all the SPPs from a single plant species.es
dc.description.sponsorshipMinisterio de Economía y Competitividad TRANSPLANTA Consolider 28317es
dc.description.sponsorshipJunta de Andalucía P08-AGR-03582 y CVI-281es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPlos One, 11 (11), e0166308-1-e0166308-19.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleCharacterization of the sucrose phosphate phosphatase (SPP) isoforms from Arabidopsis thaliana and role of the S6PPc domain in dimerizationes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Consolider28317es
dc.relation.projectIDP08-AGR-03582es
dc.relation.projectIDCVI-281es
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0166308es
dc.identifier.doi10.1371/journal.pone.0166308es
idus.format.extent19 p.es
dc.journaltitlePlos Onees
dc.publication.volumen11es
dc.publication.issue11es
dc.publication.initialPagee0166308-1es
dc.publication.endPagee0166308-19es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). España
dc.contributor.funderJunta de Andalucía

FicherosTamañoFormatoVerDescripción
Characterization of the sucrose.pdf1.961MbIcon   [PDF] Ver/Abrir  

Este registro aparece en las siguientes colecciones

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como: Attribution-NonCommercial-NoDerivatives 4.0 Internacional