Mostrar el registro sencillo del ítem

Artículo

dc.creatorGalmozzi, Carla V.es
dc.creatorFlorencio Bellido, Francisco Javieres
dc.creatorMuro Pastor, María Isabeles
dc.date.accessioned2017-03-14T13:07:02Z
dc.date.available2017-03-14T13:07:02Z
dc.date.issued2016
dc.identifier.citationGalmozzi, C.V., Florencio Bellido, F.J. y Muro Pastor, M. (2016). The cyanobacterial ribosomal-associated protein LrtA is involved in post-stress survival in synechocystis sp. PCC 6803. Plos One, 11 (7), e0159346-1-e0159346-24.
dc.identifier.issn1932-6203es
dc.identifier.urihttp://hdl.handle.net/11441/55837
dc.description.abstractA light-repressed transcript encodes the LrtA protein in cyanobacteria. We show that halflife of lrtA transcript from Synechocystis sp. PCC 6803 is higher in dark-treated cells as compared to light-grown cells, suggesting post-transcriptional control of lrtA expression. The lrtA 5' untranslated leader region is involved in that darkness-dependent regulation. We also found that Synechocystis sp. PCC 6803 LrtA is a ribosome-associated protein present in both 30S and 70S ribosomal particles. In order to investigate the function of this protein we have constructed a deletion mutant of the lrtA gene. Cells lacking LrtA (lrtA) had significantly lower amount of 70S particles and a greater amount of 30S and 50S particles, suggesting a role of LrtA in stabilizing 70S particles. Synechocystis strains with different amounts of LrtA protein: wild-type, lrtA, and LrtAS (overexpressing lrtA) showed no differences in their growth rate under standard laboratory conditions. However, a clear LrtA dose-dependent effect was observed in the presence of the antibiotic tylosin, being the LrtAS strains the most sensitive. Similar results were obtained under hyperosmotic stress caused by sorbitol. Conversely, after prolonged periods of starvation, lrtA strains were delayed in their growth with respect to the wild-type and the LrtAS strains. A positive role of LrtA protein in post-stress survival is proposed.es
dc.description.sponsorshipJunta de Andalucía P07-CVI-02792 y BIO-284es
dc.description.sponsorshipMinisterio de Economía y Competitividad y Fondo Social Europeo BFU2013-41712-Pes
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPlos One, 11 (7), e0159346-1-e0159346-24.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectChloramphenicoles
dc.subjectChlorophylles
dc.subjectErythromycines
dc.subjectLlincomycines
dc.subjectLrtA proteines
dc.subjectPuromycines
dc.subjectRibosome proteines
dc.subjectTetracyclinees
dc.titleThe cyanobacterial ribosomal-associated protein LrtA is involved in post-stress survival in synechocystis sp. PCC 6803es
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDP07-CVI-02792es
dc.relation.projectIDBIO-284es
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/BFU2013-41712-Pes
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0159346es
dc.identifier.doi10.1371/journal.pone.0159346es
idus.format.extent24 p.es
dc.journaltitlePlos Onees
dc.publication.volumen11es
dc.publication.issue7es
dc.publication.initialPagee0159346-1es
dc.publication.endPagee0159346-24es

FicherosTamañoFormatoVerDescripción
The cyanobacterial ribosamal.pdf3.497MbIcon   [PDF] Ver/Abrir  

Este registro aparece en las siguientes colecciones

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como: Attribution-NonCommercial-NoDerivatives 4.0 Internacional