dc.creator | Hidalgo Perea, Ángeles | es |
dc.creator | Margaret Oliver, Isabel María | es |
dc.creator | Crespo Rivas, Juan Carlos | es |
dc.creator | Parada Ibáñez, Maribel Eugenia | es |
dc.creator | Murdoch, Piedad del Socorro | es |
dc.creator | López, Abigail | es |
dc.creator | Buendía Clavería, Ana María | es |
dc.creator | Moreno Onorato, Francisco Javier | es |
dc.creator | Gil Serrano, Antonio Miguel | es |
dc.creator | Rodríguez Carvajal, Miguel Ángel | es |
dc.creator | Ruiz Sainz, José Enrique | es |
dc.creator | Vinardell González, José María | es |
dc.date.accessioned | 2016-04-22T10:31:01Z | |
dc.date.available | 2016-04-22T10:31:01Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 1350-0872 | es |
dc.identifier.uri | http://hdl.handle.net/11441/40310 | |
dc.description.abstract | In this work, the role of the rkpU and rkpJ genes in the production of the K-antigen
polysaccharides (KPS) and in the symbiotic capacity of Sinorhizobium fredii HH103, a broad
host-range rhizobial strain able to nodulate soybean and many other legumes, was studied. The
rkpJ- and rkpU-encoded products are orthologous to Escherichia coli proteins involved in
capsule export. S. fredii HH103 mutant derivatives were contructed in both genes. To our
knowledge, this is the first time that the role of rkpU in KPS production has been studied in
rhizobia. Both rkpJ and rkpU mutants were unable to produce KPS. The rkpU derivative also
showed alterations in its lipopolysaccharide (LPS). Neither KPS production nor rkpJ and rkpU
expression was affected by the presence of the flavonoid genistein. Soybean (Glycine max) plants
inoculated with the S. fredii HH103 rkpU and rkpJ mutants showed reduced nodulation and clear
symptoms of nitrogen starvation. However, neither the rkpJ nor the rkpU mutants were
significantly impaired in their symbiotic interaction with cowpea (Vigna unguiculata). Thus, we
demonstrate for the first time to our knowledge the involvement of the rkpU gene in rhizobial KPS
production and also show that the symbiotic relevance of the S. fredii HH103 KPS depends on
the specific bacterium–legume interaction. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Society for General Microbiology | es |
dc.relation.ispartof | Microbiology, 156, 3398–3411 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | The rkpU gene of Sinorhizobium fredii HH103 is required for bacterial K-antigen polysaccharide production and for efficient nodulation with soybean but not with cowpea | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química orgánica | es |
dc.identifier.doi | 10.1099/mic.0.042499-0 | es |
idus.format.extent | 14 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/40310 | |