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dc.creatorRodrigo, Rosa M.es
dc.creatorRendón Unceta, María del Carmenes
dc.creatorTorreblanca López, Josées
dc.creatorGarcía Herdugo, Gregorioes
dc.creatorMoreno Onorato, Francisco Javieres
dc.date.accessioned2016-04-19T10:03:25Z
dc.date.available2016-04-19T10:03:25Z
dc.date.issued1992
dc.identifier.citationRodrigo, R.M., Rendón Unceta, M.d.C., Torreblanca López, J., García Herdugo, G. y Moreno Onorato, F.J. (1992). Characterization and immunolocalization of RNA polymerase I transcription factor UBF with anti-NOR serum in protozoa, higher plant and vertebrate cells. Journal of Cell Science,103,1053-1063
dc.identifier.issn0021-9533es
dc.identifier.urihttp://hdl.handle.net/11441/40076
dc.description.abstractWe have used anti-NOR serum from a patient with rheumatoid arthritis, to study its reactivity on different phylogenetically separated species such as protozoa, higher plants, birds and mammals. The biochemical characteristics of the antigens detected after applying mono- and two-dimensional electrophoresis and electrophoretic transfers confirm that they correspond to the rRNA polymerase I transcription factor UBF. We have demonstrated the different molecular sizes, depending on the cell complexity, but the same neutral isoelectric points in whole cell extracts of the different species. We have also demonstrated an immunolocalization of this transcription factor to the fibrillar component in all the species studied. These results suggest a high conservation of UBF throughout evolution and the possibility of using this anti-NOR serum as a tool for the study of the structure, nucleolar organization and functional roles of the different nucleolar components.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherThe Company of Biologistses
dc.publisherCompany of Biologists
dc.relation.ispartofJournal of Cell Science,103,1053-1063
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAnti-NOR serumes
dc.subjectElectrophoresises
dc.subjectImmunoblottinges
dc.subjectImmunolabellinges
dc.subjectNucleolar componentses
dc.subjectNucleoluses
dc.subjectrDNA transcriptiones
dc.subjectrRNA polymerase I transcription factores
dc.titleCharacterization and immunolocalization of RNA polymerase I transcription factor UBF with anti-NOR serum in protozoa, higher plant and vertebrate cellses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Biología Celulares
dc.relation.publisherversiones
dc.identifier.idushttps://idus.us.es/xmlui/handle/11441/40076

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