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dc.creatorDelmani, Fatima Azzahra
dc.creatorTorreblanca López, José
dc.creatorMoreno Onorato, Francisco Javier
dc.creatorGarcía Herdugo, Gregorio
dc.creatorVilaplana Serrano, Rosario
dc.creatorGonzález Vílchez, Francisco
dc.date.accessioned2016-03-02T11:11:18Z
dc.date.available2016-03-02T11:11:18Z
dc.date.issued2014
dc.identifier.citationDelmani, F.A., Torreblanca López, J., Moreno Onorato, F.J., García Herdugo, G., Vilaplana Serrano, R. y González Vílchez, F. (2014). Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP). Indian Journal of Experimental Biology (IJEB), 52(6), 579-588
dc.identifier.issn0975-1009es
dc.identifier.issn0019-5189es
dc.identifier.urihttp://hdl.handle.net/11441/36721
dc.description.abstractThe interaction of a newly synthesized antitumor complex cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP) with DNA was investigated in vitro through a number of techniques including comet assay, immunoprecipitation, and immunolocalization of certain nucleolar proteins (the upstream binding factor (UBF) and fibrillarin) involved in DNA transcription, rRNA processing, and ribosomal assembly. The results showed that RAP binds to the DNA of two cell lines (H4 and Hs-683) causing a delay in cell proliferation rate leading to a number of cellular modifications. These modifications include DNA-damage assessed by the single cell gel electrophoresis method (comet assay) and variation in the expression of nucleolar proteins; UBF was more abundant in RAP treated cells, this was explained by the high affinity of this protein to DNA modified by RAP. On the other hand, fibrillarin was found in less quantities in RAP treated cells which was explained by a de-regulation of the ribosomal machinery caused by RAPes
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherNational Institute of Science Communication and Information Resourceses
dc.relation.ispartofIndian Journal of Experimental Biology (IJEB), 52(6), 579-588
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAntitumor drugses
dc.subjectDNA-damagees
dc.subjectFibrillarines
dc.subjectRAPes
dc.subjectTumor cells in vitroes
dc.subjectUBFes
dc.titleBiological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP)es
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Biología Celulares
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Química Inorgánicaes
dc.relation.publisherversionhttp://nopr.niscair.res.in/bitstream/123456789/28864/1/IJEB%2052%286%29%20579-588.pdfes
dc.identifier.idushttps://idus.us.es/xmlui/handle/11441/36721

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