Artículo
Dynamic modes of Notch transcription hubs conferring memory and stochastic activation revealed by live imaging the co-activator Mastermind
Autor/es | De Haro Arbona, F. Javier
Roussos, Charalambos Baloul, Sarah Townson, Jonathan Gómez Lamarca, Mª Jesús ![]() ![]() ![]() ![]() ![]() ![]() ![]() Bray, Sarah |
Departamento | Universidad de Sevilla. Departamento de Biología Celular |
Fecha de publicación | 2024-05-10 |
Fecha de depósito | 2024-08-26 |
Publicado en |
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Resumen | Developmental programming involves the accurate conversion of signalling levels and dynamics to transcriptional outputs. The transcriptional relay in the Notch pathway relies on nuclear complexes containing the co-activator ... Developmental programming involves the accurate conversion of signalling levels and dynamics to transcriptional outputs. The transcriptional relay in the Notch pathway relies on nuclear complexes containing the co-activator Mastermind (Mam). By tracking these complexes in real time, we reveal that they promote the formation of a dynamic transcription hub in Notch ON nuclei which concentrates key factors including the Mediator CDK module. The composition of the hub is labile and persists after Notch withdrawal conferring a memory that enables rapid reformation. Surprisingly, only a third of Notch ON hubs progress to a state with nascent transcription, which correlates with polymerase II and core Mediator recruitment. This probability is increased by a second signal. The discovery that target-gene transcription is probabilistic has far-reaching implications because it implies that stochastic differences in Notch pathway output can arise downstream of receptor activation. |
Cita | De Haro Arbona, F.J., Roussos, C., Baloul, S., Townson, J., Gómez Lamarca, M.J. y Bray, S. (2024). Dynamic modes of Notch transcription hubs conferring memory and stochastic activation revealed by live imaging the co-activator Mastermind. eLife, 12, RP92083. https://doi.org/10.7554/eLife.92083. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Dynamic modes.pdf | 6.612Mb | ![]() | Ver/ | Versión publicada |
Dynamic modes-figures-v1.pdf | 10.03Mb | ![]() | Ver/ | |