Artículo
Daidzein and Equol: Ex Vivo and In Silico Approaches Targeting COX-2, iNOS, and the Canonical Inflammasome Signaling Pathway
Autor/es | Márquez Flores, Yazmín K.
Martínez Galero, Elizdath Correa Basurto, José Sixto López, Yudibeth Villegas Lama, Isabel ![]() ![]() ![]() ![]() ![]() ![]() ![]() Rosillo Ramírez, María de los Ángeles ![]() ![]() ![]() ![]() ![]() ![]() ![]() Cárdeno Galván, Ana Alarcón de la Lastra Romero, Catalina ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Departamento | Universidad de Sevilla. Departamento de Farmacología |
Fecha de publicación | 2024-05-16 |
Fecha de depósito | 2024-06-10 |
Publicado en |
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Resumen | Background: The inflammasome is a cytosolic multiprotein complex associated with multiple autoimmune diseases. Phytochemical compounds in soy (Glycine max) foods, such as isoflavones, have been reported for their ... Background: The inflammasome is a cytosolic multiprotein complex associated with multiple autoimmune diseases. Phytochemical compounds in soy (Glycine max) foods, such as isoflavones, have been reported for their anti-inflammatory properties. Aim: the anti-inflammatory activity of DZ (daidzein) and EQ (equol) were investigated in an ex vivo model of LPS-stimulated murine peritoneal macrophages and by molecular docking correlation. Methods: Cells were pre-treated with DZ (25, 50, and 100 µM) or EQ (5, 10, and 25 µM), followed by LPS stimulation. The levels of PGE2, NO, TNF-α, IL-6, and IL-1β were analyzed by ELISA, whereas the expressions of COX-2, iNOS, NLRP3, ASC, caspase 1, and IL-18 were measured by Western blotting. Also, the potential for transcriptional modulation by targeting NF-κB, COX-2, iNOS, NLRP3, ASC, and caspase 1 was investigated by molecular docking. Results: The anti-inflammatory responses observed may be due to the modulation of NF-κB due to the binding of DZ or EQ, which is translated into decreased TNF-α, COX-2, iNOS, NLRP3, and ASC levels. Conclusion: This study establishes that DZ and EQ inhibit LPS-induced inflammatory responses in peritoneal murine macrophages via down-regulation of NO and PGE2 generation, as well as the inhibition of the canonical inflammasome pathway, regulating NLRP3, and consequently decreasing IL-1β and IL-18 activation. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España Junta de Andalucía Instituto Politécnico Nacional, Mexico Consejo Nacional de Ciencia y Tecnología (CONACYT). México |
Identificador del proyecto | AGL-2017-89342-P
![]() CTS-259 ![]() 20171085 ![]() 20181622 ![]() 20232041 ![]() 20160204 ![]() CB254600 ![]() PDCPN-782 ![]() |
Cita | Márquez Flores, Y.K., Martínez Galero, E., Correa Basurto, J., Sixto López, Y., Villegas Lama, I., Rosillo Ramírez, M.d.l.Á.,...,Alarcón de la Lastra Romero, C. (2024). Daidzein and Equol: Ex Vivo and In Silico Approaches Targeting COX-2, iNOS, and the Canonical Inflammasome Signaling Pathway. Pharmaceuticals, 17 (5), 647. https://doi.org/10.3390/ph17050647. |
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