Mostrar el registro sencillo del ítem
Artículo
Role of low-level quinolone resistance in generating tolerance in Escherichia coli under therapeutic concentrations of ciprofloxacin
dc.creator | Ortiz Padilla, Miriam | es |
dc.creator | Díaz Díaz, S. | es |
dc.creator | Machuca, J. | es |
dc.creator | Tejada-Gonzalez, A. | es |
dc.creator | Recacha, E. | es |
dc.creator | Docobo Pérez, Fernando | es |
dc.creator | Pascual Hernández, Álvaro | es |
dc.creator | Rodríguez Martínez, José Manuel | es |
dc.date.accessioned | 2024-05-28T11:52:31Z | |
dc.date.available | 2024-05-28T11:52:31Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Ortiz Padilla, M., Díaz Díaz, S., Machuca, J., Tejada-Gonzalez, A., Recacha, E., Docobo Pérez, F.,...,Rodríguez Martínez, J.M. (2020). Role of low-level quinolone resistance in generating tolerance in Escherichia coli under therapeutic concentrations of ciprofloxacin. Journal of Antimicrobial Chemotherapy, 75 (8), 2124-2132. https://doi.org/10.1093/jac/dkaa151. | |
dc.identifier.issn | 0305-7453 | es |
dc.identifier.issn | 1460-2091 | es |
dc.identifier.uri | https://hdl.handle.net/11441/159180 | |
dc.description.abstract | Background: Tolerance (including persistence) and resistance result in increased survival under antibiotic pressure. Objectives: We evaluated the interplay between resistance and tolerance to ciprofloxacin under therapeutic and killing conditions to determine the contribution of low-level quinolone resistance (LLQR) mechanisms to tolerance. We also determined how the interaction between resistance (LLQR phenotypes) and tolerance was modified under SOS response suppression. Methods: Twelve isogenic Escherichia coli strains harbouring quinolone resistance mechanisms combined with SOS response deficiency and six clinical E. coli isolates (LLQR or non-LLQR) were evaluated. Survival (tolerance or persistence) assays were used to measure surviving bacteria after a short period (up to 4 h) of bactericidal antibiotic treatment under therapeutic and killing concentrations of ciprofloxacin [1 mg/L, EUCAST/CLSI breakpoint for resistance; and 2.5 mg/L, peak serum concentration (Cmax) of this drug]. Results: QRDR substitutions (S83L in GyrA alone or combined with S80R in ParC) significantly increased the fraction of tolerant bacteria (2-4 log10 cfu/mL) after exposure to ciprofloxacin at clinically relevant concentrations. The impact on tolerant bacteria due to SOS response suppression (including persistence mediated by the tisB gene) was reversed by LLQR mechanisms at therapeutic concentrations. Furthermore, no reduction in the fraction of tolerant bacteria due to SOS response suppression was observed when S83L in GyrA plus S80R in ParC were combined. Conclusions: Tolerance and quinolone resistance mutations interact synergistically, giving LLQR mechanisms an additional role in allowing bacterial survival and evasion of therapeutic antimicrobial conditions by a combination of the two strategies. At clinically relevant concentrations, LLQR mechanisms reverse further impact of SOS response suppression in reducing bacterial tolerance. | es |
dc.format | application/pdf | es |
dc.format.extent | 9 p. | es |
dc.language.iso | eng | es |
dc.publisher | Oxford University Press | es |
dc.relation.ispartof | Journal of Antimicrobial Chemotherapy, 75 (8), 2124-2132. | |
dc.subject | Quinolones | es |
dc.subject | Tolerance | es |
dc.subject | Persistence | es |
dc.subject | Low-level resistance | es |
dc.subject | SOS response | es |
dc.title | Role of low-level quinolone resistance in generating tolerance in Escherichia coli under therapeutic concentrations of ciprofloxacin | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.publisherversion | https://academic.oup.com/jac/article/75/8/2124/5836071 | es |
dc.identifier.doi | 10.1093/jac/dkaa151 | es |
dc.journaltitle | Journal of Antimicrobial Chemotherapy | es |
dc.publication.volumen | 75 | es |
dc.publication.issue | 8 | es |
dc.publication.initialPage | 2124 | es |
dc.publication.endPage | 2132 | es |
dc.description.awardwinning | Premio Mensual Publicación Científica Destacada de la US. Facultad de Medicina |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
Role of low-level quinolone ... | 409.6Kb | [PDF] | Ver/ | |
Este registro aparece en las siguientes colecciones
Este documento está protegido por los derechos de propiedad intelectual e industrial. Sin perjuicio de las exenciones legales existentes, queda prohibida su reproducción, distribución, comunicación pública o transformación sin la autorización del titular de los derechos, a menos que se indique lo contrario.