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Artículo
Validated method for the determination of perfluorinated compounds in placental tissue samples based on a simple extraction procedure followed by ultra-high performance liquid chromatography tandem mass spectrometry analysis
dc.creator | Martín Bueno, Julia | es |
dc.creator | Rodríguez-Gómez, Rocío | es |
dc.creator | Zafra-Gómez, Alberto | es |
dc.creator | Alonso Álvarez, Esteban | es |
dc.creator | Vílchez, José Luis | es |
dc.creator | Navalón, Alberto | es |
dc.date.accessioned | 2024-05-06T09:59:26Z | |
dc.date.available | 2024-05-06T09:59:26Z | |
dc.date.issued | 2016-04 | |
dc.identifier.issn | 0039-9140 | es |
dc.identifier.issn | 1873-3573 | es |
dc.identifier.uri | https://hdl.handle.net/11441/157666 | |
dc.description.abstract | Xenobiotic exposure during pregnancy is inevitable. Determination of perfluorinated compounds (PFCs), chemicals described as environmental contaminants by Public Health Authorities due to their persistence, bioaccumulation and toxicity, is a challenge. In the present work, a method based on a simplified sample treatment involving freeze–drying, solvent extraction and dispersive clean-up of the extracts using C₁₈ sorbents followed by an ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) analysis was developed and validated for the determination of five perfluorinated carboxylic acids (C₄–C₈) and perfluorooctane sulfonate (PFOS) in placental tissue samples. The most influential parameters affecting the extraction method and clean-up were optimized using Design of Experiments (DOE). The method was validated using matrix-matched calibration. Found limits of detection (LODs) ranged from 0.03 to 2 ng g⁻¹ and limits of quantification (LOQs) from 0.08 to 6 ng g⁻¹, while inter- and intra-day variability was under 14% in all cases. Recovery rates for spiked samples ranged from 94% to 113%. The method was satisfactorily applied for the determination of compounds in human placental tissue samples collected at delivery from 25 randomly selected women. | es |
dc.format | application/pdf | es |
dc.format.extent | 46 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Perfluorinated compounds | es |
dc.subject | Placental tissue | es |
dc.subject | Dispersive solid phase extraction | es |
dc.subject | UHPLC–MS/MS | es |
dc.title | Validated method for the determination of perfluorinated compounds in placental tissue samples based on a simple extraction procedure followed by ultra-high performance liquid chromatography tandem mass spectrometry analysis | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Analítica | es |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/abs/pii/S0039914015305427 | es |
dc.identifier.doi | 10.1016/j.talanta.2015.12.020 | es |
dc.contributor.group | Universidad de Sevilla. FQM344: Análisis Químico Industrial y Medioambiental | es |
dc.journaltitle | Talanta | es |
dc.publication.volumen | 150 | es |
dc.publication.initialPage | 169 | es |
dc.publication.endPage | 176 | es |
Ficheros | Tamaño | Formato | Ver | Descripción |
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T_martin-bueno_2016_validated.pdf | 1.703Mb | ![]() | Ver/ | Postprint. Peer reviewed and Accepted Version |