dc.creator | Salas Lloret, Daniel | es |
dc.creator | Jansen, Nicolette S. | es |
dc.creator | Nagamalleswari, Easa | es |
dc.creator | van der Meulen, Coen | es |
dc.creator | Gracheva, Ekaterina | es |
dc.creator | de Ru, Arnoud H. | es |
dc.creator | Otte, H. Anne Marie | es |
dc.creator | van Veelen, Peter A. | es |
dc.creator | Pichler, Andrea | es |
dc.creator | Goedhart, Joachim | es |
dc.creator | Vertegaal, Alfred C.O. | es |
dc.creator | González Prieto, Román | es |
dc.date.accessioned | 2024-02-15T15:11:52Z | |
dc.date.available | 2024-02-15T15:11:52Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Salas Lloret, D., Jansen, N.S., Nagamalleswari, E., van der Meulen, C., Gracheva, E., de Ru, A.H.,...,González Prieto, R. (2023). SUMO-activated Target Traps (SATTs) Enable the Identification of a Comprehensive E3-specific SUMO Proteome. Science Advances, 9 (31), eadh2073. https://doi.org/10.1126/sciadv.adh2073. | |
dc.identifier.issn | 2375-2548 | es |
dc.identifier.uri | https://hdl.handle.net/11441/155280 | |
dc.description.abstract | Ubiquitin and ubiquitin-like conjugation cascades consist of dedicated E1, E2, and E3 enzymes with E3s providing substrate specificity. Mass spectrometry–based approaches have enabled the identification of more than 6500 SUMO2/3 target proteins. The limited number of SUMO E3s provides the unique opportunity to systematically study E3 substrate wiring. We developed SUMO-activated target traps (SATTs) and systematically identified substrates for eight different SUMO E3s, PIAS1, PIAS2, PIAS3, PIAS4, NSMCE2, ZNF451, LAZSUL (ZNF451-3), and ZMIZ2. SATTs enabled us to identify 427 SUMO1 and 961 SUMO2/3 targets in an E3-specific manner. We found pronounced E3 substrate preference. Quantitative proteomics enabled us to measure substrate specificity of E3s, quantified using the SATT index. Furthermore, we developed the Polar SATTs web-based tool to browse the dataset in an interactive manner. Overall, we uncover E3-to-target wiring of 1388 SUMO substrates, highlighting unique and overlapping sets of substrates for eight different SUMO E3 ligases. | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID2021-122361NA-I00 | es |
dc.description.sponsorship | Junta de Andalucía EMERGIA20_00276 | es |
dc.description.sponsorship | Dutch Cancer Society KWF-KIG 11367/2017-2 | es |
dc.description.sponsorship | European Research Council 310913 | es |
dc.description.sponsorship | Swiss National Science Foundation 310030_204583 | es |
dc.format | application/pdf | es |
dc.format.extent | 16 p. | es |
dc.language.iso | eng | es |
dc.publisher | American Association for the Advancement of Science | es |
dc.relation.ispartof | Science Advances, 9 (31), eadh2073. | |
dc.rights | Atribución-NoComercial 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.title | SUMO-activated Target Traps (SATTs) Enable the Identification of a Comprehensive E3-specific SUMO Proteome | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.relation.projectID | PID2021-122361NA-I00 | es |
dc.relation.projectID | EMERGIA20_00276 | es |
dc.relation.projectID | KWF-KIG 11367/2017-2 | es |
dc.relation.projectID | 310913 | es |
dc.relation.projectID | 724.016.003 | es |
dc.relation.projectID | 310030_204583 | es |
dc.relation.publisherversion | https://dx.doi.org/10.1126/sciadv.adh2073 | es |
dc.identifier.doi | 10.1126/sciadv.adh2073 | es |
dc.journaltitle | Science Advances | es |
dc.publication.volumen | 9 | es |
dc.publication.issue | 31 | es |
dc.publication.initialPage | eadh2073 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | Junta de Andalucía | es |
dc.contributor.funder | Dutch Cancer Society | es |
dc.contributor.funder | European Research Council (ERC) | es |
dc.contributor.funder | Dutch Research Council | es |
dc.contributor.funder | Swiss National Science Foundation (SNFS) | es |
dc.description.awardwinning | Premio Anual Publicación Científica Destacada de la US. Facultad de Biología | |