Artículo
GunA of Sinorhizobium (Ensifer) fredii HH103 is a T3SS-secreted cellulase that differentially affects symbiosis with cowpea and soybean
Autor/es | Jiménez Guerrero, Irene
Pérez Montaño, Francisco de Asís Zdyb, Anna Beutler, Mandy Werner, Gesa Göttfert, Michael Ollero Márquez, Francisco Javier Vinardell González, José María López Baena, Francisco Javier |
Departamento | Universidad de Sevilla. Departamento de Microbiología |
Fecha de publicación | 2019-11-12 |
Fecha de depósito | 2024-02-12 |
Publicado en |
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Resumen | Aims The symbiosis between Sinorhizobium fredii HH103 and its host legumes is influenced by the type 3 secretion system (T3SS), which delivers proteins (effectors) directly into the host cells to promote infection. GunA, ... Aims The symbiosis between Sinorhizobium fredii HH103 and its host legumes is influenced by the type 3 secretion system (T3SS), which delivers proteins (effectors) directly into the host cells to promote infection. GunA, one of the predicted HH103 effectors, potentially codes for a cellulase. In this work we tried to characterise GunA and elucidate its role in symbosis with soybean and cowpea. Methods A GunA::HA fusion protein was constructed to study T3SS-dependent secretion. Cellulase activity of GunA was measured and gunA::uidA-GFP and gunA::cyA fusions were constructed to monitor gunA expression in nodules and to study translocation to the host cells, respectively. Finally, the symbiotic performance of a gunA mutant was studied in soybean and cowpea. |
Cita | Jiménez Guerrero, I., Pérez Montaño, F.d.A., Zdyb, A., Beutler, M., Werner, G., Göttfert, M.,...,López Baena, F.J. (2019). GunA of Sinorhizobium (Ensifer) fredii HH103 is a T3SS-secreted cellulase that differentially affects symbiosis with cowpea and soybean. Plant Soil, 435, 15-26. https://doi.org/10.1007/s11104-018-3875-3. |
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