Artículo
Helicobacter ibis sp. nov., isolated from faecal droppings of black-faced ibis (Theristicus melanopis)
Autor/es | López Cantillo, Mónica
Vidal Veuthey, Boris Mella, Armin Ruiz de la Haba, Rafael Collado, Luis |
Departamento | Universidad de Sevilla. Departamento de Microbiología y Parasitología |
Fecha de publicación | 2023 |
Fecha de depósito | 2024-01-16 |
Publicado en |
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Resumen | As part of a larger study on Epsilonproteobacteria carried by wild birds in the city of Valdivia (southern Chile), two curved rod-shaped Gram-stain-negative strains (A82T and WB-40) were recovered from faecal samples and ... As part of a larger study on Epsilonproteobacteria carried by wild birds in the city of Valdivia (southern Chile), two curved rod-shaped Gram-stain-negative strains (A82T and WB-40) were recovered from faecal samples and subjected to a taxonomic study. Results of a genus-specific PCR showed that these isolates belonged to the genus Helicobacter. Further identification by 16S rRNA and hsp60 (60 kDa heat-shock protein) gene sequence analysis revealed that they formed a separate phylogenetic clade, different from other known Helicobacter species with ‘Helicobacter burdigaliensis’ CNRCH 2005/566HT and Helicobacter valdiviensis WBE14T being the most closely related species. This was confirmed by core-genome phylogeny as well as digital DNA–DNA hybridization and average nucleotide identity analyses between the genomes of strains A82T and WB-40 and all other Helicobacter species. The draft genome sequences of A82T and WB-40, obtained by Illumina NextSeq 2000 sequencing, consisted of 1.6 Mb with a G+C content of 31.9–32.0 mol%. The results obtained from the phylogenetic and genomic characteri-zation, together with their different morphological and biochemical features, revealed that these two strains represent a novel species, for which we propose the name Helicobacter ibis sp. nov. with A82T (=LMG 32718T=CCCT 22.04T) as the type strain. |
Agencias financiadoras | Comisión Nacional de Investigación Científica y Tecnológica (CONICYT). Chile Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía |
Identificador del proyecto | 1200125
PID2020-118136GB-I00 P20_01066 BIO-213 |
Cita | López Cantillo, M., Vidal Veuthey, B., Mella, A., Ruiz de la Haba, R. y Collado, L. (2023). Helicobacter ibis sp. nov., isolated from faecal droppings of black-faced ibis (Theristicus melanopis). International Journal of Systematic and Evolutionary Microbiology, 73 (11), 005983. https://doi.org/10.1099/ijsem.0.005983. |
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