Artículo
Natrinema salsiterrestre sp. nov., an extremely halophilic archaeon isolated from a hypersaline soil
Autor/es | Strakova, Dasa
Sánchez-Porro Álvarez, Cristina Ruiz de la Haba, Rafael Ventosa Ucero, Antonio |
Departamento | Universidad de Sevilla. Departamento de Microbiología y Parasitología |
Fecha de publicación | 2023-08-01 |
Fecha de depósito | 2024-01-16 |
Publicado en |
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Resumen | An extremely halophilic archaeal strain, designated S1CR25-10T, was isolated from hypersaline soil sampled in the Odiel Salt-marshes Natural Area in Southwestern Spain (Huelva) and subjected to a polyphasic taxonomic ... An extremely halophilic archaeal strain, designated S1CR25-10T, was isolated from hypersaline soil sampled in the Odiel Salt-marshes Natural Area in Southwestern Spain (Huelva) and subjected to a polyphasic taxonomic characterization. The cells were Gram-stain-negative, motile and their colonies were pink-pigmented. It was a strictly aerobic haloarchaeon that could grow at 25–55 °C (optimum, 37 °C), at pH 6.0–9.0 (optimum, pH 7.0–8.0) and in the presence of 12–30 % (w/v) total salts (optimum, 20–25 %, w/v). The phylogenetic analysis based on the comparison of the 16S rRNA gene sequences revealed that strain S1CR25-10T belongs to the genus Natrinema, with 98.9 % similarity to Natrinema salinisoli SLN56T. In addition, the values of orthologous average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity were below the threshold limits accepted for prokaryotic species delineation, with N. salinisoli SLN56T showing the highest relatedness values (92.6 % and 48.4 %, respectively). The major polar lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a glycolipid chromatographically identical to sulfated diglycosyl diether. The DNA G+C content of the isolate was 63.8 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic characterization and the whole genome results, strain S1CR25-10T represents a new species within the genus Natrinema, for which the name Natrinema salsiterrestre sp. nov., with type strain S1CR25-10T (=CECT 30623T=CCM 9251T), is proposed. |
Agencias financiadoras | Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía |
Identificador del proyecto | ID2020-118136GB-I00
P20_01066 BIO-213 |
Cita | Strakova, D., Sánchez-Porro Álvarez, C., Ruiz de la Haba, R. y Ventosa Ucero, A. (2023). Natrinema salsiterrestre sp. nov., an extremely halophilic archaeon isolated from a hypersaline soil. International Journal of Systematic and Evolutionary Microbiology, 73 (8), 005960. https://doi.org/10.1099/ijsem.0.005960. |
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