Effect of Zn binding to phytate and humic substances on its uptake by wheat (Triticum durum L.) as affected by carbonates and Fe oxides

Abstract

Although complexation with soil organic matter may improve zinc (Zn) bioavailability to plants, the effect of Zn sorbent surface on the use of complexed Zn by plants remains unknown. The objective of this research was to elucidate how Zn complexation with humic substances (HS) and phytate affects the uptake of Zn by wheat plants depending on the main sorbent surface in growth media, i.e., carbonates and Fe oxides. To this end, two pot experiments were performed, one using Fe oxide-coated siliceous as the siliceous growth medium sand and the other using a mixture of calcareous sand and siliceous sand as the calcareous growth medium. Each experiment involved three Zn sources, Zn-HS complex, Zn phytate, and ZnSO4. All sources were applied with surface irrigation at two Zn rates (0.25 and 2 mg kg−1 growth medium). The Zn-HS complex significantly increased Zn uptake by plants in both media, relative to the other two Zn sources, but no significant difference was observed between Zn phytate and ZnSO4. In the calcareous medium, Zn-HS complex and Zn phytate resulted in significantly higher dry biomass yields of wheat than ZnSO4. In the siliceous medium, spike and shoot dry biomass yields with Zn-HS complex at the low rate and Zn phytate at both rates were not significantly different from those with ZnSO4 at the high rate. After harvest, approximately 50% of the Zn applied as Zn-HS complex remained extractable by diethylenetriaminepentaacetic acid (DTPA), while this proportion was less than 20% for the other Zn sources. Thus, Zn-HS complex and Zn phytate are sources of available Zn for plants, and they are more effective than ZnSO4 in increasing plant growth, particularly when carbonates are the main Zn sorbent surface.