Article
Molecular recognition between guanine and cytosine-functionalized nucleolipid hybrid bilayers supported on gold (111) electrodes
Author/s | Álvarez Malmagro, Julia
Su, Zhangfei Leitch, J. Jay Prieto Dapena, Francisco Rueda Rueda, Manuela Lipkowski, Jacek |
Department | Universidad de Sevilla. Departamento de Química Física |
Publication Date | 2020-04 |
Deposit Date | 2023-12-21 |
Published in |
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Abstract | A hybrid bilayer lipid membrane (hBLM), constructed with a 1-hexadecanethiol self-assembled interior leaflet and a 1,2-dipalmitoyl-sn-glycero-3-cytidine nucleolipid exterior leaflet, was deposited at the surface of a gold ... A hybrid bilayer lipid membrane (hBLM), constructed with a 1-hexadecanethiol self-assembled interior leaflet and a 1,2-dipalmitoyl-sn-glycero-3-cytidine nucleolipid exterior leaflet, was deposited at the surface of a gold (111) electrode. This system was used to investigate the molecular recognition reaction between the cytosine moieties of the lipid head group with guanine molecules in the bulk electrolyte solution. Electrochemical measurements and photon polarization modulation infrared reflection absorption spectroscopy (PMIRRAS) were employed to characterize the system and determine the extent of the molecular recognition reaction. The capacitance of the hBLM-covered gold electrode was very low (~1 μF cm−2), therefore the charge density at the gold surface was small. Changing the electrode potential had a minimal effect on the complexation between the cytosine moieties and guanine molecules due to small changes in the static electric field across the membrane. This behavior favored the formation of the guanine–cytosine complex. |
Funding agencies | Junta de Andalucía Ministerio de Economía y Competitividad (MINECO). España |
Project ID. | FQM202
CTQ2014-57515-C2-1-R |
Citation | Alvarez Malmagro, J., Su, Z., Leitch, J.J., Prieto Dapena, F., Rueda Rueda, M. y Lipkowski, J. (2020). Molecular recognition between guanine and cytosine-functionalized nucleolipid hybrid bilayers supported on gold (111) electrodes. Bioelectrochemistry, 132, 107416. https://doi.org/10.1016/j.bioelechem.2019.107416. |
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