dc.creator | Selvais, Camille M. | es |
dc.creator | De Cock, Laura L. | es |
dc.creator | Brichard, Sonia M. | es |
dc.creator | Davis López de Carrizosa, María América | es |
dc.date.accessioned | 2023-12-20T08:44:18Z | |
dc.date.available | 2023-12-20T08:44:18Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Selvais, C.M., De Cock, L.L., Brichard, S.M. y Davis López de Carrizosa, M.A. (2022). Fiber type and subcellular-specific analysis of lipid droplet content in skeletal muscle. Journal of Visualized Experiments (JoVE), 2022 (184), e63718. https://doi.org/10.3791/63718. | |
dc.identifier.issn | 1940-087X | es |
dc.identifier.uri | https://hdl.handle.net/11441/152720 | |
dc.description.abstract | Skeletal muscle lipid infiltration, known as myosteatosis, increases with obesity and
ageing. Myosteatosis has also recently been discovered as a negative prognostic
factor for several other disorders such as cardiovascular disease and cancer.
Excessive lipid infiltration decreases muscle mass and strength. It also results in
lipotoxicity and insulin resistance depending on total intramyocellular lipid content,
lipid droplet (LD) morphology, and subcellular distribution. Fiber type (oxidative vs
glycolytic) is also important, since oxidative fibers have a greater capacity to utilize
lipids. Because of their crucial implications in pathophysiology, in-depth studies on LD
dynamics and function in a fiber type-specific manner are warranted.
Herein, a complete protocol is presented for the quantification of intramyocellular
lipid content and analysis of LD morphology and subcellular distribution in a fiber
type-specific manner. To this end, serial muscle cryosections were stained with the
fluorescent dye Bodipy and antibodies against myosin heavy chain isoforms. This
protocol enables the simultaneous processing of different muscles, saving time and
avoiding possible artifacts and, thanks to a personalized macro created in Fiji, the
automatization of LD analysis is also possible. | es |
dc.description.sponsorship | Fonds National de la Recherche Scientifique de Bélgica - FNRS-CJ.0022.20 | es |
dc.description.sponsorship | Société Francophone du Diabète (SFD-Roche Diabetes Care) | es |
dc.format | application/pdf | es |
dc.format.extent | 28 p. | es |
dc.language.iso | eng | es |
dc.publisher | MyJove Corporation | es |
dc.relation.ispartof | Journal of Visualized Experiments (JoVE), 2022 (184), e63718. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Fiber type and subcellular-specific analysis of lipid droplet content in skeletal muscle | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología | es |
dc.relation.projectID | FNRS-CJ.0022.20 | es |
dc.relation.publisherversion | https://dx.doi.org/10.3791/63718 | es |
dc.identifier.doi | 10.3791/63718 | es |
dc.journaltitle | Journal of Visualized Experiments (JoVE) | es |
dc.publication.volumen | 2022 | es |
dc.publication.issue | 184 | es |
dc.publication.initialPage | e63718 | es |
dc.contributor.funder | Fonds National de la Recherche Scientifique (FNRS). Bélgica | es |
dc.contributor.funder | Société Francophone du Diabète (SFD) | es |