Dataset
Study the immunotoxic effect of cylindrospermopsin [Dataset]
Autor/es | Casas Rodríguez, Antonio
Cebadero Domínguez, Óscar Puerto Rodríguez, María Cameán Fernández, Ana María Jos Gallego, Ángeles Mencía |
Compilador de datos | Casas Rodríguez, Antonio |
Gestor de datos | Puerto Rodríguez, María |
Contacto | Cameán Fernández, Ana María |
Departamento | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal |
Idioma (ISO) | inglés (eng) |
Fecha de difusión | 2023-12-20 |
Fecha de depósito | 2023-12-20 |
Fecha de creación | 2022 |
Resumen | Cylindrospermopsin (CYN) is a cyanotoxin with an increasing occurrence, and therefore
it is important to elucidate its toxicity profile. CYN has been classified as a cytotoxin, although the scientific literature has already ... Cylindrospermopsin (CYN) is a cyanotoxin with an increasing occurrence, and therefore it is important to elucidate its toxicity profile. CYN has been classified as a cytotoxin, although the scientific literature has already revealed that it affects a wide range of organs and systems. However, research on its potential immunotoxicity is still limited. Thus, this study aimed to evaluate the impact of CYN on two human cell lines representative of the immune system: THP-1 (monocytes) and Jurkat (lymphocytes). CYN reduced cell viability, leading to mean effective concentrations (EC50 24 h) of 6.00± 1.04 µM and 5.20± 1.20 µM for THP-1 and Jurkat cells, respectively, and induced cell death mainly by apoptosis in both experimental models. Moreover, CYN decreased the differentiation of monocytes to macrophages after 48 h of exposure. In addition, an up-regulation of the mRNA expression of different cytokines, such as interleukin (IL) 2, IL-8, tumor necrosis factor-alpha (TNF-α) and interferon-gamma (INF-ɣ), was also observed mainly after 24 h exposure in both cell lines. However, only an increase in TNF-_ in THP-1 supernatants was observed by ELISA. Overall, these results suggest the immunomodulatory activity of CYN in vitro. Therefore, further research is required to evaluate the impact of CYN on the human immune system. Dataset contains the numerical values of the results obtained regarding cytotoxicity, differentiation, cell death mechanisms (apoptosis/necrosis), gene expression and interleukin leakage. |
Contenido | Numerical data of the results of cytotoxicity, differentiation, cell death mechanisms (apoptosis/necrosis), gene expression and interleukin leakage |
Agencias financiadoras | Ministerio de Ciencia e Innovación (MICIN). España |
Identificador del proyecto | PID 2019-104890RB-I00 |
Asociado a la publicación | Casas-Rodríguez, A., Cebadero-Dominguez, Ó., Puerto, M., Cameán, A. M., & Jos, A. (2023). Immunomodulatory Effects of Cylindrospermopsin in Human T Cells and Monocytes. Toxins, 15(4), 301. https://doi.org/10.3390/toxins15040301 |
Cita | Casas Rodríguez, A., Cebadero Domínguez, Ó.,...,Jos Gallego, Á.M.. Study the immunotoxic effect of cylindrospermopsin [Dataset]. idUS (Depósito de Investigación de la Universidad de Sevilla). https://doi.org/10.12795/11441/152718 |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Casas-Rodríguez et al., 2023 ... | 26.13Kb | [Microsoft Excel 2007] | Acceso restringido. Petición a través del formulario. | |
readme_Casas Rodríguez et al_2023 ... | 5.200Kb | [Fichero de texto] | Ver/ | Fichero readme.txt |