Datos de Investigación (Nutrición y Bromatología, Toxicología y Medicina Legal )
URI permanente para esta colecciónhttps://hdl.handle.net/11441/150658
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Dataset Anti-VEGF Effect of Bioactive Indolic Compounds and Hydroxytyrosol Metabolites [Dataset](2024-01-30) Gallardo Fernández, Marta; Cerezo López, Ana Belén; Hornedo Ortega, Ruth; Troncoso González, Ana María; García Parrilla, María del Carmen; Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal; Cerezo López, Ana Belén; Ministerio de Ciencia, Innovación y Universidades (MICINN). España; Universidad de Sevilla. AGR 167: Derivados de la uvaEl dasaset incluye los datos brutos de los experimentos western blot y ELISA realizados en el trabajo titulado "Anti-VEGF Effect of Bioactive Indolic Compounds and Hydroxytyrosol Metabolites". También se incluyen las medias y las desviaciones estándar.
Dataset Study the immunotoxic effect of reduced graphene [Dataset](2023-12-20) Cebadero Domínguez, Óscar; Casas Rodríguez, Antonio; Puerto Rodríguez, María; Cameán Fernández, Ana María; Jos Gallego, Ángeles Mencía; Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal; Jos Gallego, Ángeles Mencía; Cebadero Domínguez, Óscar; Jos Gallego, Ángeles Mencía; Junta de Andalucía; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Universidad de Sevilla. CTS358: ToxicologíaThe cytotoxicity response of reduced graphene oxide (rGO) was investigated in monocytes (THP-1) and human T cells (Jurkat). A mean effective concentration (EC50-24 h) of 121.45 ± 11.39 μg/mL and 207.51 ± 21.67 μg/mL for cytotoxicity was obtained in THP-1 and Jurkat cells, respectively. rGO decreased THP-1 monocytes differentiation at the highest concentration after 48 h of exposure. Regarding the inflammatory response at genetic level, changes (up and downregulations) were observed depending on the cell line, the exposure time and the interleukins investigated. Apoptosis/ necrosis genes expression was not altered in THP-1 cells but in Jurkat cells BAX and BCL-2 were downregulated after 4h. Finally, rGO did not trigger a significant release of any cytokine at any exposure time assayed. Dataset contains the numerical values of the results obtained regarding cytotoxicity, differentiation, gene expression and interleukin leakage.Dataset Genotoxicity evaluation of graphene in vitro assays [dataset](2023-12-20) Cebadero Domínguez, Óscar; Medrano Padial, Concepción; Puerto Rodríguez, María; Sánchez Ballester, Soraya; Cameán Fernández, Ana María; Jos Gallego, Ángeles Mencía; Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal; Puerto Rodríguez, María; Cebadero Domínguez, Óscar; Puerto Rodríguez, María; Junta de Andalucía; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Universidad de Sevilla. CTS358: ToxicologíaThe aim of this study was to assess the potential mutagenicity and genotoxicity of graphene oxide (GO) and reduced-graphene oxide (rGO) following the recommendations of the European Food Safety Authority (EFSA). Thus, the mouse lymphoma assay (MLA) and the micronucleus test (MN) were performed in L5178YTk ± cells, and the Caco-2 cells were used for the standard and modified comet assays. The results indicated that GO (0–250 μg/mL) was not mutagenic in the MLA. However, rGO revealed mutagenic activity from 250 μg/mL and 125 μg/mL after 4h and 24h of exposure, respectively. In the MN test, negative results were obtained for both compounds at the concentrations assayed (0–250 μg/mL) for GO/rGO. Moreover, no DNA strand breaks, or oxidative DNA damage were detected in Caco-2 cells exposed to GO (0–250 μg/mL) and rGO (0–176.3 μg/mL for 24h and 0–166.5 μg/mL for 48h). Dataset contains the numerical values of the results obtained regarding to MLA assay 4 and 24 h, MN test 24h and comet assay 24 and 48h.