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dc.creatorElander, Pernilla H.es
dc.creatorHolla, Sanjanaes
dc.creatorSabljic, Igores
dc.creatorGutiérrez Beltrán, Emilioes
dc.creatorWillems, Patrickes
dc.creatorBozhkov, Peter V.es
dc.creatorMinina, Elena A.es
dc.date.accessioned2023-09-04T14:19:13Z
dc.date.available2023-09-04T14:19:13Z
dc.date.issued2023
dc.identifier.citationElander, P.H., Holla, S., Sabljic, I., Gutiérrez Beltrán, E., Willems, P., Bozhkov, P.V. y Minina, E.A. (2023). Interactome of Arabidopsis ATG5 Suggests Functions beyond Autophagy. International Journal of Molecular Sciences, 24 (15), 12300. https://doi.org/10.3390/ijms241512300.
dc.identifier.issn1661-6596es
dc.identifier.issn1422-0067es
dc.identifier.urihttps://hdl.handle.net/11441/148613
dc.description.abstractAutophagy is a catabolic pathway capable of degrading cellular components ranging from individual molecules to organelles. Autophagy helps cells cope with stress by removing superfluous or hazardous material. In a previous work, we demonstrated that transcriptional upregulation of two autophagy-related genes, ATG5 and ATG7, in Arabidopsis thaliana positively affected agronomically important traits: biomass, seed yield, tolerance to pathogens and oxidative stress. Although the occurrence of these traits correlated with enhanced autophagic activity, it is possible that autophagy-independent roles of ATG5 and ATG7 also contributed to the phenotypes. In this study, we employed affinity purification and LC-MS/MS to identify the interactome of wild-type ATG5 and its autophagy-inactive substitution mutant, ATG5K128R Here we present the first interactome of plant ATG5, encompassing not only known autophagy regulators but also stress-response factors, components of the ubiquitin-proteasome system, proteins involved in endomembrane trafficking, and potential partners of the nuclear fraction of ATG5. Furthermore, we discovered post-translational modifications, such as phosphorylation and acetylation present on ATG5 complex components that are likely to play regulatory functions. These results strongly indicate that plant ATG5 complex proteins have roles beyond autophagy itself, opening avenues for further investigations on the complex roles of autophagy in plant growth and stress responses.es
dc.description.sponsorshipSwedish Research Council Formas 2021-01812, 2017-00541es
dc.description.sponsorshipCarl Tryggers Foundation CTS 20:287es
dc.description.sponsorshipMinisterio de Ciencia e Innovación PID2020–119737GA–I00es
dc.description.sponsorshipJunta de Andalucía ProyExcel_00587es
dc.formatapplication/pdfes
dc.format.extent16 p.es
dc.language.isoenges
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)es
dc.relation.ispartofInternational Journal of Molecular Sciences, 24 (15), 12300.
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAutophagy-unrelated functionses
dc.subjectEndomembrane traffickinges
dc.subjectHXK1es
dc.subjectNuclear ATG12es
dc.subjectNuclear ATG5es
dc.subjectPlant proteomicses
dc.subjectPlant ubiquitin-like conjugation systemes
dc.subjectPosttranslational modificationses
dc.subjectPP2Aes
dc.subjectProteasomees
dc.titleInteractome of Arabidopsis ATG5 Suggests Functions beyond Autophagyes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectID2021-01812es
dc.relation.projectID2017-00541es
dc.relation.projectIDCTS 20:287es
dc.relation.projectIDPID2020–119737GA–I00es
dc.relation.projectIDProyExcel_00587es
dc.relation.publisherversionhttps://doi.org/10.3390/ijms241512300es
dc.identifier.doi10.3390/ijms241512300es
dc.journaltitleInternational Journal of Molecular Scienceses
dc.publication.volumen24es
dc.publication.issue15es
dc.publication.initialPage12300es
dc.contributor.funderSwedish Research Council Formas. Swedenes
dc.contributor.funderCarl Tryggers Foundationes
dc.contributor.funderMinisterio de Ciencia e Innovación (MICIN). Españaes
dc.contributor.funderJunta de Andalucíaes

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