Por motivos de mantenimiento se ha deshabilitado el inicio de sesión temporalmente. Rogamos disculpen las molestias.
Artículo
Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer
Autor/es | Vázquez Carretero, María Dolores
García Miranda, Pablo Balda, María S. Matter, Karl Ilundáin Larrañeta, María Anunciación Ana Peral Rubio, María José |
Departamento | Universidad de Sevilla. Departamento de Fisiología |
Fecha de publicación | 2021 |
Fecha de depósito | 2023-07-05 |
Publicado en |
|
Resumen | We reported that Disabled-2 (Dab2) is located at the apical membrane in suckling rat intestine. Here, we discovered that, in colon of suckling and adult mouse and of adult human, Dab2 is only at lateral crypt cell membrane ... We reported that Disabled-2 (Dab2) is located at the apical membrane in suckling rat intestine. Here, we discovered that, in colon of suckling and adult mouse and of adult human, Dab2 is only at lateral crypt cell membrane and colocalized with E-cadherin. Dab2 depletion in Caco-2 cells led to E-cadherin internalization indicating that its membrane location requires Dab2. In mice, we found that 3 days of dextran sulfate sodium-induced colitis increased Dab2/E-cadherin colocalization, which was decreased as colitis progressed to 6 and 9 days. In agreement, Dab2/E-cadherin colocalization increased in human mild and severe ulcerative colitis and in polyps, being reduced in colon adenocarcinomas, which even showed epithelial Dab2 absence and E-cadherin delocalization. Epithelial Dab2 decrement preceded that of E-cadherin. We suggest that Dab2, by inhibiting E-cadherin internalization, stabilizes adherens junctions, and its absence from the epithelium may contribute to development of colon inflammation and cancer. |
Agencias financiadoras | Junta de Andalucía |
Identificador del proyecto | CTS 5884 |
Cita | Vázquez Carretero, M.D., García Miranda, P., Balda, M.S., Matter, K., Ilundáin Larrañeta, M.A.A. y Peral Rubio, M.J. (2021). Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer. Journal of Cellular Physiology, 236 (2), 1083-1093. https://doi.org/10.1002/jcp.29917. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
Dab2-Cadh para iDUS.pdf | 2.206Mb | [PDF] | Ver/ | Versión aceptada |