dc.creator | Holmes, Susannah | es |
dc.creator | Kirkwood, Henry J. | es |
dc.creator | Bean, Richard | es |
dc.creator | Giewekemeyer, Klaus | es |
dc.creator | Martin, Andrew V. | es |
dc.creator | Hadian-Jazi, Marjan | es |
dc.creator | Cruz-Mazo, Francisco | es |
dc.creator | Gañán-Calvo, Alfonso M. | es |
dc.creator | Darmanin, Connie | es |
dc.date.accessioned | 2023-06-19T16:02:54Z | |
dc.date.available | 2023-06-19T16:02:54Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 2041-1723 | es |
dc.identifier.uri | https://hdl.handle.net/11441/147334 | |
dc.description | This article is licensed under a Creative Commons
Attribution 4.0 International License, which permits use, sharing,
adaptation, distribution and reproduction in any medium or format, as
long as you give appropriate credit to the original author(s) and the
source, provide a link to the Creative Commons license, and indicate if
changes were made. The images or other third party material in this
article are included in the article’s Creative Commons license, unless
indicated otherwise in a credit line to the material. If material is not
included in the article’s Creative Commons license and your intended
use is not permitted by statutory regulation or exceeds the permitted
use, you will need to obtain permission directly from the copyright
holder. To view a copy of this license, visit http://creativecommons.org/
licenses/by/4.0/. | es |
dc.description.abstract | The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light
Source (LCLS) II are extremely intense sources of X-rays capable of generating
Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition
rates. Previous work has shown that it is possible to use consecutive X-ray
pulses to collect diffraction patterns from individual crystals. Here, we exploit
the MHz pulse structure ofthe European XFEL to obtain two complete datasets
from the same lysozyme crystal, first hit and the second hit, before it exits the
beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution
structures. Comparisons between the two structures reveal no indications of
radiation damage or significant changes within the active site, consistent with
the calculated dose estimates. This demonstrates MHz SFX can be used as a
tool for tracking sub-microsecond structural changes in individual single
crystals, a technique we refer to as multi-hit SFX. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 p. | es |
dc.language.iso | eng | es |
dc.publisher | Springer Nature | es |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Ingeniería Aeroespacial y Mecánica de Fluidos | es |
dc.relation.projectID | CE140100011 | es |
dc.relation.projectID | PID2019-108278RB | es |
dc.relation.projectID | P18- FR-3375 | es |
dc.relation.publisherversion | https://www.nature.com/articles/s41467-022-32434-6 | es |
dc.identifier.doi | 10.1038/s41467-022-32434-6 | es |
dc.contributor.group | Universidad de Sevilla. TEP219: Física de Fluidos y Microfluidica | es |
dc.journaltitle | Nature Communications | es |
dc.publication.volumen | 13 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 4708 | es |
dc.contributor.funder | Centro del Consejo de Investigación de Australia | es |
dc.contributor.funder | Agencia Estatal de Investigación. España | es |
dc.contributor.funder | Junta de Andalucía | es |