dc.creator | Montserrat de la Paz, Sergio | es |
dc.creator | Carrillo-Berdasco, Gabriela | es |
dc.creator | Rivero-Pino, Fernando | es |
dc.creator | Villanueva-Lazo, Álvaro | es |
dc.creator | Millán-Linares, María del Carmen | es |
dc.date.accessioned | 2023-06-13T13:30:30Z | |
dc.date.available | 2023-06-13T13:30:30Z | |
dc.date.issued | 2022-12-27 | |
dc.identifier.citation | Montserrat de la Paz, S., Carrillo-Berdasco, G., Rivero-Pino, F., Villanueva-Lazo, Á. y Millán-Linares, M.d.C. (2022). Hemp protein hydrolysates modulate inflammasome-related genes in microglial cells. Biology, 12 (1), 49. https://doi.org/10.3390/biology12010049. | |
dc.identifier.issn | 2079-7737 | es |
dc.identifier.uri | https://hdl.handle.net/11441/147172 | |
dc.description.abstract | A prolonged inflammatory response can lead to the development of neurodegenerative
diseases such as Alzheimer’s disease. Enzymatic hydrolysis is a sustainable way to increase the
value of protein sources by obtaining peptides that can exert bioactivity. Hemp (Cannabis sativa L.)
protein hydrolysates have been proven to exert anti-inflammatory activity. In this study, two hemp
protein hydrolysate (HPHs), obtained with Alcalase as sole catalyst, or with Alcalase followed by
Flavourzyme, were evaluated as inflammatory mediators (TNFα, IL-1β, IL-6, and IL-10), microglial
polarization markers (Ccr7, iNos, Arg1, and Ym1), and genes related to inflammasome activation
(Nlrp3, Asc, Casp1, and Il18), employing the lipopolysaccharide (LPS)-induced neuroinflammation
model in murine BV-2 microglial cells. A significant decrease of the expression of proinflammatory
genes (e.g., Tnfα, Ccr7, inos, and Nlrp3, among others) and increase of the expression anti-inflammatory
cytokines in microglial cells was observed after treatment with the test HPHs. This result in the cell
model suggests a polarization toward an anti-inflammatory M2 phenotype. Our results show that the
evaluated HPHs show potential neuroprotective activity in microglial cells via the inflammasome. | es |
dc.format | application/pdf | es |
dc.format.extent | 16 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Biology, 12 (1), 49. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Neuroinflammation | es |
dc.subject | Microglia | es |
dc.subject | Inflammasome | es |
dc.subject | Hemp peptides | es |
dc.subject | Immunonutrition | es |
dc.title | Hemp protein hydrolysates modulate inflammasome-related genes in microglial cells | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología | es |
dc.relation.projectID | P20_00661 | es |
dc.relation.publisherversion | https://www.mdpi.com/2079-7737/12/1/49 | es |
dc.identifier.doi | 10.3390/biology12010049 | es |
dc.journaltitle | Biology | es |
dc.publication.volumen | 12 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 49 | es |
dc.contributor.funder | Plan Andaluz de Investigación, Desarrollo e Innovación. España | es |