dc.creator | Koropouli, Eleftheria | es |
dc.creator | Wang, Qiang | es |
dc.creator | Mejías Estévez, Rebeca María | es |
dc.creator | Hand, Randal | es |
dc.creator | Wang, Tao | es |
dc.creator | Ginty, David D. | es |
dc.creator | Kolodkin, Alex L. | es |
dc.date.accessioned | 2023-06-07T18:17:05Z | |
dc.date.available | 2023-06-07T18:17:05Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Koropouli, E., Wang, Q., Mejías Estévez, R.M., Hand, R., Wang, T., Ginty, D.D. y Kolodkin, A.L. (2023). Palmitoylation regulates neuropilin-2 localization and function in cortical neurons and conveys specificity to semaphorin signaling via palmitoyl acyltransferases. eLife, 12. https://doi.org/10.7554/eLife.83217. | |
dc.identifier.issn | 2050-084X | es |
dc.identifier.uri | https://hdl.handle.net/11441/147018 | |
dc.description.abstract | Secreted semaphorin 3F (Sema3F) and semaphorin 3A (Sema3A) exhibit remarkably distinct effects on deep layer excitatory cortical pyramidal neurons; Sema3F mediates dendritic spine pruning, whereas Sema3A promotes the elaboration of basal dendrites. Sema3F and Sema3A signal through distinct holoreceptors that include neuropilin-2 (Nrp2)/plexinA3 (PlexA3) and neuropi-lin-1 (Nrp1)/PlexA4, respectively. We find that Nrp2 and Nrp1 are S-palmitoylated in cortical neurons and that palmitoylation of select Nrp2 cysteines is required for its proper subcellular localization, cell surface clustering, and also for Sema3F/Nrp2-dependent dendritic spine pruning in cortical neurons, both in vitro and in vivo. Moreover, we show that the palmitoyl acyltransferase ZDHHC15 is required for Nrp2 palmitoylation and Sema3F/Nrp2-dependent dendritic spine pruning, but it is dispensable for Nrp1 palmitoylation and Sema3A/Nrp1-dependent basal dendritic elaboration. Therefore, palmi-toyl acyltransferase-substrate specificity is essential for establishing compartmentalized neuronal structure and functional responses to extrinsic guidance cues. | es |
dc.description.sponsorship | National Institutes of Health MH100024-Project #3, R21NS085358 | es |
dc.format | application/pdf | es |
dc.format.extent | 34 p. | es |
dc.language.iso | eng | es |
dc.publisher | eLife Sciences Publications | es |
dc.relation.ispartof | eLife, 12. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Palmitoylation regulates neuropilin-2 localization and function in cortical neurons and conveys specificity to semaphorin signaling via palmitoyl acyltransferases | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología | es |
dc.relation.projectID | MH100024-Project #3 | es |
dc.relation.projectID | R21NS085358 | es |
dc.relation.publisherversion | https://doi.org/10.7554/eLife.83217 | es |
dc.identifier.doi | 10.7554/eLife.83217 | es |
dc.journaltitle | eLife | es |
dc.publication.volumen | 12 | es |
dc.contributor.funder | National Institutes of Health | es |