dc.creator | Figueroa Bossi, Nara | es |
dc.creator | Sánchez Romero, María Antonia | es |
dc.creator | Kerboriou, Patricia | es |
dc.creator | Naquin, Delphine | es |
dc.creator | Mendes, Clara | es |
dc.creator | Bouloc, Philippe | es |
dc.creator | Casadesus Pursals, Josep | es |
dc.creator | Bossi, Lionello | es |
dc.date.accessioned | 2023-05-30T11:52:09Z | |
dc.date.available | 2023-05-30T11:52:09Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Figueroa Bossi, N., Sánchez Romero, M.A., Kerboriou, P., Naquin, D., Mendes, C., Bouloc, P.,...,Bossi, L. (2022). Pervasive transcription enhances the accessibility of H-NS–silenced promoters and generates bistability in Salmonella virulence gene expression. Proceedings of the National Academy of Sciences (PNAS), 119 (30), e2203011119. https://doi.org/10.1073/pnas.2203011119. | |
dc.identifier.issn | 0027-8424 | es |
dc.identifier.issn | 1091-6490 | es |
dc.identifier.uri | https://hdl.handle.net/11441/146778 | |
dc.description.abstract | In Escherichia coli and Salmonella, many genes silenced by the nucleoid structuring protein H-NS are activated upon inhibiting Rho-dependent transcription termination.
This response is poorly understood and difficult to reconcile with the view that H-NS
acts mainly by blocking transcription initiation. Here we have analyzed the basis for
the up-regulation of H-NS–silenced Salmonella pathogenicity island 1 (SPI-1) in cells
depleted of Rho-cofactor NusG. Evidence from genetic experiments, semiquantitative
50 rapid amplification of complementary DNA ends sequencing (5’ RACE-Seq), and
chromatin immunoprecipitation sequencing (ChIP-Seq) shows that transcription originating from spurious antisense promoters, when not stopped by Rho, elongates into a
H-NS–bound regulatory region of SPI-1, displacing H-NS and rendering the DNA
accessible to the master regulator HilD. In turn, HilD’s ability to activate its own transcription triggers a positive feedback loop that results in transcriptional activation of the
entire SPI-1. Significantly, single-cell analyses revealed that this mechanism is largely
responsible for the coexistence of two subpopulations of cells that either express or do
not express SPI-1 genes. We propose that cell-to-cell differences produced by stochastic
spurious transcription, combined with feedback loops that perpetuate the activated
state, can generate bimodal gene expression patterns in bacterial populations. | es |
dc.description.sponsorship | Agence Nationale de la Recherche de Francia - ANR-15-CE11-0024-03 | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación y Agencia Estatal de Investigación, de España y Fondo Regional Europeo - PID2020-116995RB-I00 | es |
dc.format | application/pdf | es |
dc.format.extent | 9 p. | es |
dc.language.iso | eng | es |
dc.publisher | National Academy of Sciences | es |
dc.relation.ispartof | Proceedings of the National Academy of Sciences (PNAS), 119 (30), e2203011119. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | pervasive transcription | es |
dc.subject | H-NS | es |
dc.subject | silencing | es |
dc.subject | pathogenicity islands | es |
dc.subject | bistability | es |
dc.title | Pervasive transcription enhances the accessibility of H-NS–silenced promoters and generates bistability in Salmonella virulence gene expression | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología y Parasitología | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | |
dc.relation.projectID | ANR-15-CE11-0024-03 | es |
dc.relation.projectID | PID2020-116995RB-I00 | es |
dc.relation.publisherversion | https://doi.org/10.1073/pnas.2203011119 | es |
dc.identifier.doi | 10.1073/pnas.2203011119 | es |
dc.journaltitle | Proceedings of the National Academy of Sciences (PNAS) | es |
dc.publication.volumen | 119 | es |
dc.publication.issue | 30 | es |
dc.publication.initialPage | e2203011119 | es |
dc.contributor.funder | Agence Nationale de la Recherche. France | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | Agencia Estatal de Investigación. España | es |