dc.creator | García-Fernández, Noelia | es |
dc.creator | Macher, Hada C. | es |
dc.creator | Suárez Artacho, Gonzalo | es |
dc.creator | Gómez Bravo, Miguel Ángel | es |
dc.creator | Molinero Hueso, Patrocinio | es |
dc.creator | Guerrero Montávez, Juan Miguel | es |
dc.creator | Rubio Calvo, Amalia Macarena | es |
dc.date.accessioned | 2023-04-17T13:17:50Z | |
dc.date.available | 2023-04-17T13:17:50Z | |
dc.date.issued | 2022-12-22 | |
dc.identifier.citation | García-Fernández, N., Macher, H.C., Suárez Artacho, G., Gómez Bravo, M.Á., Molinero Hueso, P., Guerrero Montávez, J.M. y Rubio Calvo, A.M. (2022). Donor-specific cell-free DNA qPCR quantification as a noninvasive accurate biomarker for early rejection detection in liver transplantation. JOURNAL OF CLINICAL MEDICINE, 12 (36). https://doi.org/10.3390/jcm12010036. | |
dc.identifier.issn | 2077-0383 | es |
dc.identifier.uri | https://hdl.handle.net/11441/144506 | |
dc.description.abstract | Background: Graft-cell-free DNA (cfDNA) in the circulation of liver transplant recipients
has been proposed as a noninvasive biomarker of organ rejection. The aim of this study was to detect
donor-specific cfDNA (ds-cfDNA) in the recipient’s serum after either liver damage or rejection
using a qPCR-based method. (2) Methods: We proposed a qPCR method based on the amplification
of 10 specific insertion–deletion (InDel) polymorphisms to detect donor-specific circulating DNA
diluted in the recipient cfDNA. ds-cfDNA from 67 patients was evaluated during the first month
post-transplantation. (3) Results: Graft rejection in the first month post-transplantation was reported
in 13 patients. Patients without liver complications showed a transitory increase in ds-cfDNA levels
at transplantation. Patients with rejection showed significant differences in ds-cfDNA increase over
basal levels at both the rejection time point and several days before rejection. Receiver operator
characteristic (ROC) analysis showed that ds-cfDNA levels discriminated rejection, with an AUC of
0.96. Maximizing both sensitivity and specificity, a threshold cutoff of 8.6% provided an estimated
positive and negative predictive value of 99% and 60%, respectively. (4) Conclusions: These results
suggest that ds-cfDNA may be a useful marker of graft integrity in liver transplant patients to screen
for rejection and liver damage. | es |
dc.description.sponsorship | Consejería de Salud de la Junta de Andalucía | es |
dc.format | application/pdf | es |
dc.format.extent | 14 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | JOURNAL OF CLINICAL MEDICINE, 12 (36). | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Donor-specific cfDNA | es |
dc.subject | Liver transplantation | es |
dc.subject | Graft rejection | es |
dc.subject | Noninvasive biomarker | es |
dc.title | Donor-specific cell-free DNA qPCR quantification as a noninvasive accurate biomarker for early rejection detection in liver transplantation | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Cirugía | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología | es |
dc.relation.projectID | PI0047/2014 | es |
dc.relation.publisherversion | https://www.mdpi.com/2077-0383/12/1/36 | es |
dc.identifier.doi | 10.3390/jcm12010036 | es |
dc.journaltitle | JOURNAL OF CLINICAL MEDICINE | es |
dc.publication.volumen | 12 | es |
dc.publication.issue | 36 | es |