dc.creator | Borroni, Davide | es |
dc.creator | Paytuví-Gallart, Andreu | es |
dc.creator | Sanseverino, Walter | es |
dc.creator | Gómez-Huertas, Carmen | es |
dc.creator | Bonci, Paola | es |
dc.creator | Romano, Vito | es |
dc.creator | Rocha de Lossada, Carlos | es |
dc.date.accessioned | 2023-03-28T13:16:58Z | |
dc.date.available | 2023-03-28T13:16:58Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Borroni, D., Paytuví-Gallart, A., Sanseverino, W., Gómez-Huertas, C., Bonci, P., Romano, V. y Rocha de Lossada, C. (2022). Exploring the Healthy Eye Microbiota Niche in a Multicenter Study. International Journal of Molecular Sciences, 23 (18), 10229. https://doi.org/10.3390/ijms231810229. | |
dc.identifier.issn | 1422-0067 | es |
dc.identifier.uri | https://hdl.handle.net/11441/143645 | |
dc.description.abstract | Purpose: This study aims to explore and characterize healthy eye microbiota. Methods:
Healthy subjects older than 18 years were selected for this descriptive cross-sectional study. Samples
were collected with an eSwab with 1 mL of Liquid Amies Medium (Copan Brescia, Italy). Following
DNA extraction, libraries preparation, and amplification, PCR products were purified and end repaired for barcode ligation. Libraries were pooled to a final concentration of 26 pM. Template
preparation was performed with Ion Chef according to Ion 510, Ion 520, and Ion 530 Kit-Chef protocol.
Sequencing of the amplicon libraries was carried out on a 520 or 530 chip using the Ion Torrent S5
system (Thermo Fisher; Waltham, MA, USA). Raw reads were analyzed with GAIA (v 2.02). Results:
Healthy eye microbiota is a low-diversity microbiome. The vast majority of the 137 analyzed samples
were highly enriched with Staphylococcus, whereas only in a few of them, other genera such as Bacillus,
Pseudomonas, and Corynebacterium predominate. We found an average of 88 genera with an average
Shannon index of 0.65. Conclusion: We identified nine different ECSTs. A better understanding of
healthy eye microbiota has the potential to improve disease diagnosis and personalized regimens to
promote health. | es |
dc.format | application/pdf | es |
dc.format.extent | 11 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI AG | es |
dc.relation.ispartof | International Journal of Molecular Sciences, 23 (18), 10229. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Microbiome | es |
dc.subject | Healthy | es |
dc.subject | Metagenomics | es |
dc.subject | Eye | es |
dc.subject | 16 | es |
dc.subject | OSDI | es |
dc.title | Exploring the Healthy Eye Microbiota Niche in a Multicenter Study | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Cirugía | es |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/23/18/10229 | es |
dc.identifier.doi | 10.3390/ijms231810229 | es |
dc.journaltitle | International Journal of Molecular Sciences | es |
dc.publication.volumen | 23 | es |
dc.publication.issue | 18 | es |
dc.publication.initialPage | 10229 | es |