Artículo
Exploring the Healthy Eye Microbiota Niche in a Multicenter Study
Autor/es | Borroni, Davide
Paytuví-Gallart, Andreu Sanseverino, Walter Gómez-Huertas, Carmen Bonci, Paola Romano, Vito Rocha de Lossada, Carlos ![]() ![]() ![]() ![]() ![]() |
Departamento | Universidad de Sevilla. Departamento de Cirugía |
Fecha de publicación | 2022 |
Fecha de depósito | 2023-03-28 |
Publicado en |
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Resumen | Purpose: This study aims to explore and characterize healthy eye microbiota. Methods:
Healthy subjects older than 18 years were selected for this descriptive cross-sectional study. Samples
were collected with an eSwab ... Purpose: This study aims to explore and characterize healthy eye microbiota. Methods: Healthy subjects older than 18 years were selected for this descriptive cross-sectional study. Samples were collected with an eSwab with 1 mL of Liquid Amies Medium (Copan Brescia, Italy). Following DNA extraction, libraries preparation, and amplification, PCR products were purified and end repaired for barcode ligation. Libraries were pooled to a final concentration of 26 pM. Template preparation was performed with Ion Chef according to Ion 510, Ion 520, and Ion 530 Kit-Chef protocol. Sequencing of the amplicon libraries was carried out on a 520 or 530 chip using the Ion Torrent S5 system (Thermo Fisher; Waltham, MA, USA). Raw reads were analyzed with GAIA (v 2.02). Results: Healthy eye microbiota is a low-diversity microbiome. The vast majority of the 137 analyzed samples were highly enriched with Staphylococcus, whereas only in a few of them, other genera such as Bacillus, Pseudomonas, and Corynebacterium predominate. We found an average of 88 genera with an average Shannon index of 0.65. Conclusion: We identified nine different ECSTs. A better understanding of healthy eye microbiota has the potential to improve disease diagnosis and personalized regimens to promote health. |
Cita | Borroni, D., Paytuví-Gallart, A., Sanseverino, W., Gómez-Huertas, C., Bonci, P., Romano, V. y Rocha de Lossada, C. (2022). Exploring the Healthy Eye Microbiota Niche in a Multicenter Study. International Journal of Molecular Sciences, 23 (18), 10229. https://doi.org/10.3390/ijms231810229. |
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