Artículo
Genomic study and lipidomic bioassay of Leeuwenhoekiella parthenopeia: A novel rare biosphere marine bacterium that inhibits tumor cell viability
Autor/es | Gattoni, Giuliano
Ruiz de la Haba, Rafael Martín, Jesús Reyes, Fernando Sánchez-Porro Álvarez, Cristina Feola, Antonia Zuchegna, Candida Guerrero-Flores, Shaday Varcamonti, Mario Ricca, Ezio Selem-Mojica, Nelly Ventosa Ucero, Antonio Corral, Paulina |
Departamento | Universidad de Sevilla. Departamento de Microbiología y Parasitología |
Fecha de publicación | 2023 |
Fecha de depósito | 2023-01-23 |
Publicado en |
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Resumen | The fraction of low-abundance microbiota in the marine environment is a promising target for discovering new bioactive molecules with pharmaceutical applications. Phenomena in the ocean such as diel vertical migration (DVM) ... The fraction of low-abundance microbiota in the marine environment is a promising target for discovering new bioactive molecules with pharmaceutical applications. Phenomena in the ocean such as diel vertical migration (DVM) and seasonal dynamic events influence the pattern of diversity of marine bacteria, conditioning the probability of isolation of uncultured bacteria. In this study, we report a new marine bacterium belonging to the rare biosphere, Leeuwenhoekiella parthenopeia sp. nov. Mr9T, which was isolated employing seasonal and diel sampling approaches. Its complete characterization, ecology, biosynthetic gene profiling of the whole genus Leeuwenhoekiella, and bioactivity of its extract on human cells are reported. The phylogenomic and microbial diversity studies demonstrated that this bacterium is a new and rare species, barely representing 0.0029% of the bacterial community in Mediterranean Sea metagenomes. The biosynthetic profiling of species of the genus Leeuwenhoekiella showed nine functionally related gene cluster families (GCF), none were associated with pathways responsible to produce known compounds or registered patents, therefore revealing its potential to synthesize novel bioactive compounds. In vitro screenings of L. parthenopeia Mr9T showed that the total lipid content (lipidome) of the cell membrane reduces the prostatic and brain tumor cell viability with a lower effect on normal cells. The lipidome consisted of sulfobacin A, WB 3559A, WB 3559B, docosenamide, topostin B-567, and unknown compounds. Therefore, the bioactivity could be attributed to any of these individual compounds or due to their synergistic effect. Beyond the rarity and biosynthetic potential of this bacterium, the importance and novelty of this study is the employment of sampling strategies based on ecological factors to reach the hidden microbiota, as well as the use of bacterial membrane constituents as potential novel therapeutics. Our findings open new perspectives on cultivation and the relationship between bacterial biological membrane components and their bioactivity in eukaryotic cells, encouraging similar studies in other members of the rare biosphere. |
Agencias financiadoras | Fondazione CON IL SUD. Italia Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía |
Identificador del proyecto | 2018-PDR-00533
PID2020-118136GB-I00 P-20_01066 |
Cita | Gattoni, G., Ruiz de la Haba, R., Martín, J., Reyes, F., Sánchez-Porro Álvarez, C., Feola, A.,...,Corral, P. (2023). Genomic study and lipidomic bioassay of Leeuwenhoekiella parthenopeia: A novel rare biosphere marine bacterium that inhibits tumor cell viability. Frotiers in Microbiology, 13, 1090197. https://doi.org/10.3389/fmicb.2022.1090197. |
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