dc.creator | Cruz Chamorro, Iván | es |
dc.creator | Santos Sánchez, Guillermo | es |
dc.creator | Bollati, Carlotta | es |
dc.creator | Bartolomei, Martina | es |
dc.creator | Li, Jianqiang | es |
dc.creator | Arnoldi, Anna | es |
dc.creator | Lammi, Carmen | es |
dc.date.accessioned | 2022-12-13T15:41:02Z | |
dc.date.available | 2022-12-13T15:41:02Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Cruz Chamorro, I., Santos Sánchez, G., Bollati, C., Bartolomei, M., Li, J., Arnoldi, A. y Lammi, C. (2022). Hempseed (Cannabis sativa) Peptides WVSPLAGRT and IGFLIIWV Exert Anti-inflammatory Activity in the LPS-Stimulated Human Hepatic Cell Line. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 70 (2), 577-583. https://doi.org/10.1021/acs.jafc.1c07520. | |
dc.identifier.issn | 0021-8561 | es |
dc.identifier.issn | 1520-5118 | es |
dc.identifier.uri | https://hdl.handle.net/11441/140409 | |
dc.description.abstract | WVSPLAGRT (H2) and IGFLIIWV (H3) are two transepithelial transported intestinal peptides obtained from the
hydrolysis of hempseed protein with pepsin, which exert antioxidant activity in HepG2 cells. Notably, both peptides reduce the
H2O2-induced reactive oxygen species, lipid peroxidation, and nitric oxide (NO) production levels in HepG2 cells via the
modulation of the nuclear factor erythroid 2-related factor 2 and the inducible nitric oxide synthase (iNOS) pathways, respectively.
Due to the close link between inflammation and oxidative stress and with the objective of fostering the multifunctional behavior of
bioactive peptides, in this study, the molecular characterization of the anti-inflammatory and immunomodulatory properties of H2
and H3 was carried out in HepG2 cells. In fact, both peptides were shown to modulate the production of pro (IFN-γ: −33.0 ± 6.7%
H2, p = 0.011; −13.1 ± 2.0% H3, p = <0.0001; TNF: −17.6 ± 1.7% H2, p = 0.0004; −20.3 ± 1.7% H3, p = <0.0001; and IL-6:
−15.1 ± 6.5% H3, p = 0.010)- and anti (IL-10: +9.6 ± 3.1% H2, p = 0.010; +26.0 ± 2.3% H3, p = < 0.0001)-inflammatory cytokines
and NO (−9.0 ± 0.7% H2, p = <0.0001; −7.2 ± 1.8% H3, p = <0.0001) through regulation of the NF-κB and iNOS pathways,
respectively, in HepG2 cells stimulated by lipopolysaccharides. | es |
dc.format | application/pdf | es |
dc.format.extent | 7 p. | es |
dc.language.iso | eng | es |
dc.publisher | AMER CHEMICAL SOC | es |
dc.relation.ispartof | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 70 (2), 577-583. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Food peptides | es |
dc.subject | Hempseed | es |
dc.subject | Inflammation | es |
dc.subject | Oxidative stress | es |
dc.subject | NF-κB | es |
dc.title | Hempseed (Cannabis sativa) Peptides WVSPLAGRT and IGFLIIWV Exert Anti-inflammatory Activity in the LPS-Stimulated Human Hepatic Cell Line | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología | es |
dc.relation.publisherversion | https://pubs.acs.org/doi/10.1021/acs.jafc.1c07520 | es |
dc.identifier.doi | 10.1021/acs.jafc.1c07520 | es |
dc.contributor.group | Universidad de Sevilla. CTS160: Neuroendocrinología Molecular. | es |
dc.journaltitle | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | es |
dc.publication.volumen | 70 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | 577 | es |
dc.publication.endPage | 583 | es |
dc.contributor.funder | Junta de Andalucía | es |
dc.contributor.funder | Ministerio de Educación, Cultura y Deporte | es |