dc.creator | Salguero-Aranda, Carmen | es |
dc.creator | Beltran Povea, Amparo | es |
dc.creator | Postigo-Corrales, Fátima | es |
dc.creator | Hitos, Ana Belén | es |
dc.creator | Díaz, Irene | es |
dc.creator | Caballano-Infantes, Estefanía | es |
dc.creator | Cahuana, Gladys M. | es |
dc.date.accessioned | 2022-10-26T13:11:45Z | |
dc.date.available | 2022-10-26T13:11:45Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Salguero-Aranda, C., Beltran Povea, A., Postigo-Corrales, F., Hitos, A.B., Díaz, I., Caballano-Infantes, E. y Cahuana, G.M. (2022). Pdx1 Is Transcriptionally Regulated by EGR-1 during Nitric Oxide-Induced Endoderm Differentiation of Mouse Embryonic Stem Cells. International Journal of Molecular Sciences, 23 (7), 3920. https://doi.org/10.3390/ijms23073920. | |
dc.identifier.issn | 1422-0067 | es |
dc.identifier.uri | https://hdl.handle.net/11441/138378 | |
dc.description.abstract | The transcription factor, early growth response-1 (EGR-1), is involved in the regulation of
cell differentiation, proliferation, and apoptosis in response to different stimuli. EGR-1 is described
to be involved in pancreatic endoderm differentiation, but the regulatory mechanisms controlling
its action are not fully elucidated. Our previous investigation reported that exposure of mouse
embryonic stem cells (mESCs) to the chemical nitric oxide (NO) donor diethylenetriamine nitric
oxide adduct (DETA-NO) induces the expression of early differentiation genes such as pancreatic and
duodenal homeobox 1 (Pdx1). We have also evidenced that Pdx1 expression is associated with the
release of polycomb repressive complex 2 (PRC2) and P300 from the Pdx1 promoter; these events were
accompanied by epigenetic changes to histones and site-specific changes in the DNA methylation.
Here, we investigate the role of EGR-1 on Pdx1 regulation in mESCs. This study reveals that EGR-1
plays a negative role in Pdx1 expression and shows that the binding capacity of EGR-1 to the Pdx1
promoter depends on the methylation level of its DNA binding site and its acetylation state. These
results suggest that targeting EGR-1 at early differentiation stages might be relevant for directing
pluripotent cells into Pdx1-dependent cell lineages. | es |
dc.format | application/pdf | es |
dc.format.extent | 14 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI AG | es |
dc.relation.ispartof | International Journal of Molecular Sciences, 23 (7), 3920. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | EGR-1 | es |
dc.subject | Pdx1 | es |
dc.subject | Nitric oxide | es |
dc.subject | MESCs; endoderm differentiation | es |
dc.title | Pdx1 Is Transcriptionally Regulated by EGR-1 during Nitric Oxide-Induced Endoderm Differentiation of Mouse Embryonic Stem Cells | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica | es |
dc.relation.projectID | FI11/00301 | es |
dc.relation.projectID | PI105/2010 | es |
dc.relation.projectID | TCMR06/009 | es |
dc.relation.projectID | PI-0022 | es |
dc.relation.projectID | CTS-7127/2011) | es |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/23/7/3920 | es |
dc.identifier.doi | 10.3390/ijms23073920 | es |
dc.journaltitle | International Journal of Molecular Sciences | es |
dc.publication.volumen | 23 | es |
dc.publication.issue | 7 | es |
dc.publication.initialPage | 3920 | es |
dc.contributor.funder | Instituto de Salud Carlos III | es |
dc.contributor.funder | Consejería de Igualdad, Salud y Políticas Sociales, Junta de Andalucía | es |
dc.contributor.funder | Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía | es |
dc.contributor.funder | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) | es |
dc.contributor.funder | Servicio Andaluz de Salud | es |